阔叶箬竹竹叶中黄酮碳苷的提取及酸水解过程研究  

Extraction and Acid Hydrolysis Process of C-glycosyflavone From the Leaves of Indocalamus latifolius

作　　者：李盼盼 程贤[1,2] 李胜男 陈玉湘 王衍彬[3] 毕良武 LI Panpan;CHENG Xian;LI Shengnan;CHEN Yuxiang;WANG Yanbin;BI Liangwu(Institute of Chemical Industry of Forest Products,CAF,Key Lab.of Biomass Energy and Material,Jiangsu Province,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass,Nanjing 210042,China;Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing 210037,China;Zhejiang Academy of Forestry,Hangzhou 310023,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏南京210042 [2]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037 [3]浙江省林业科学研究院,浙江杭州310023

出　　处：《林产化学与工业》2025年第2期125-132,共8页Chemistry and Industry of Forest Products

基　　金：浙江省省院合作林业科技项目(2022SY07);国家自然科学基金青年项目(32201512);广西创新驱动发展专项资金项目(桂科AA20302021-7)。

摘　　要：以阔叶箬竹竹叶为原料,首先采用超声波辅助乙醇浸提法得到箬竹叶提取物,同时建立箬竹叶提取物中牡荆苷、异牡荆苷、荭草苷、异荭草苷4种黄酮碳苷成分的高效液相色谱(HPLC)分析方法;然后对提取物进行酸水解,考察酸水解pH值、时间和温度等因素对4种黄酮碳苷总量的影响,并借助HPLC-TOF-MS对箬竹叶提取物酸水解前后的化学成分进行全表征,初步探究箬竹叶提取物在酸水解过程中的化学成分变化规律。结果表明:箬竹叶提取物中4种黄酮碳苷的总质量分数为0.92%;水解温度为80℃、pH值为2的条件下水解1.5 h后,样品中4种黄酮碳苷的总质量分数提高到1.16%,相对于水解前提高26.09%。HPLC-TOF-MS在箬竹叶提取物中可以检测到28种化合物,包括8种黄酮碳苷类化合物、 4种黄酮氧苷类化合物、 9种木脂素类化合物,以及7种其他化合物。水解后样品中可以检测到20种化合物,其中8种黄酮苷类化合物的一级质谱信号强度明显降低,甚至检测不到,但4个主要的黄酮碳苷的强度有所增加。分析发现,箬竹提取物中黄酮苷类化合物在酸水解过程中主要发生了氧苷键和碳苷键断裂,最易转化为异牡荆苷,从而促进黄酮碳苷含量的提高。Taking the leaves of Indocalamus latifolius as the raw material,the extract of I.latifolius leaves was initially obtained by the ultrasonic-assisted ethanol extraction method.Simultaneously,a high performance liquid chromatography(HPLC)method was established to analyze 4 flavonoid carbon glycosides(vitexin,isovitexin,orientin,and isoorientin)in the estracts of I.latifolius leaves.Then,the extract was hydrolyzed by acid and the effects of pH value,time,and temperature on the total content of 4 flavonoids were investigated.The chemical components of the leaves extracts before and after acid hydrolysis were fully characterized by HPLC-TOF-MS,and the chemical composition changes of the extract of I.latifolius leaves during acid hydrolysis were preliminarily explored.The results showed that the total mass fraction of 4 flavonoid glycosides in the leaves of I.latifolius was 0.92%.After hydrolysis at 80℃and pH value of 2 for 1.5 h,the total mass fraction of 4 main flavonoid glycosides in the sample increased to 1.16%,which was 26.09%higher than that before hydrolysis.HPLC-TOF-MS detected 28 compounds,including 8 flavonoid carbosides,4 flavonoid oxysides,9 lignans,and 7 other compounds in the leaves extracts of I.latifolius.20 compounds could be detected in the samples after hydrolysis.Among them,the intensity of 8 flavonoid glycosides was significantly reduced and even undetectable by mass spectrometry,while the intensity of 4 main flavonoid carbon glycosides increased.In the process of acid hydrolysis,the oxyglycoside bond and carboglycoside bond were broken in the flavonoid glycoside compounds in the extract,and were most likely to be converted into isovitexin,thus promoting the increase of flavonoid glycoside content.

关 键 词：阔叶箬竹 黄酮碳苷 超声波辅助提取 酸水解 HPLC-MS 

分 类 号：TQ35[化学工程] R284.2[医药卫生—中药学]