他汀类抑制剂对大豆疫霉的生物活性及与靶标互作分析  

Bioactivity of statin inhibitors against Phytophthora sojae and molecular docking analysis with target

作　　者：徐继涛 李秀 黄启凤 高祯 李欣航 王瑞龙 杨新宇 XU Ji-tao;LI Xiu;HUANG Qi-feng;GAO Zhen;LI Xin-hang;WANG Rui-long;YANG Xin-yu(College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《中国油料作物学报》2025年第2期461-470,共10页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划(2023YFD1401000);辽宁省种质创新藏粮于技专项计划(2023JH1/10200008);辽宁省教育厅基本科研项目(JYTMS20231277)。

摘　　要：大豆疫霉Phytophthora sojae引起的大豆根腐病是生产中的严重土传病害之一。本文通过菌丝生长速率法测定氟伐他汀、辛伐他汀、阿托伐他汀三种抑制剂对大豆疫霉的生物活性;评价了三种抑制剂对大豆疫霉孢子囊、游动孢子、卵孢子和致病能力的影响;通过分子对接预测三种抑制剂与大豆疫霉靶标PsHMGR的结合模式。结果表明:在40~200μg/mL浓度处理下,三种抑制剂均抑制大豆疫霉菌丝生长;在40μg/mL浓度处理下,抑制大豆疫霉孢子囊和卵孢子产量、休止孢萌发、促进游动孢子休止,其中阿托伐他汀抑制大豆疫霉产孢活性较氟伐他汀和辛伐他汀强;氟伐他汀和辛伐他汀抑制大豆疫霉菌丝生长活性较阿托伐他汀强;在40μg/mL浓度下,三种物质使致病能力平均降低23.9%、39.6%和69.7%。三种抑制剂与靶标PsHMGR通过疏水作用、氢键、卤键、π-π堆积和π-阳离子等形成互作。Soybean Phytophthora root rot(PRR)is an infamous soil borne disease caused by Phytophthora sojae,resulting in enormous economic losses in soybean production.In this research,we determined the sensitivity of P.sojae to 3 statin inhibitors,fluvastatin,simvastatin and atorvastatin,by mycelial growth rate method.Additionally we evaluated the effect of 3 statin inhibitors on sporangia and oospores production,zoospores encystment and germination and pathogenicity of P.sojae;and predicted the inhibitors interaction with PsHMGR target by molecular docking.Results demonstrated that these 3 statin inhibitors significantly suppress P.sojae mycelial growth within a concentration range of 40-200μg/mL,decrease sporangia and oospores production,promote zoospore encystment,the pathogenicity was decreased by 23.9%,39.6% and 69.7% at the concentration of 40μg/mL.Activity of atorvastatin on spore production was higher than fluvastatin and simvastatin,while the activity of fluvastatin and simvastatin on mycelial growth was higher than atorvastatin.The 3 inhibitors interact with conserved amino acids of PsHMGR through hydrophobic interaction,hydrogen bond,halogen bond,π-π stacking,and π-cation interactions.

关 键 词：大豆疫霉 大豆根腐病 他汀类抑制剂 生物活性 分子对接 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]