猕猴桃根部源分散泛菌葡萄糖异构酶异源表达研究  

Study on Heterologous Expression of Glucose Isomerase from Pantoea dispersa of Kiwifruit Root Origin

作　　者：智勇 夏菠 ZHI Yong;XIA Bo(College of Food Science and Technology,Hunan Agricultural University,Changsha 410000,China)

机构地区：[1]湖南农业大学食品科学技术学院,湖南长沙410000

出　　处：《中国果菜》2025年第4期35-40,共6页China Fruit & Vegetable

基　　金：湖南省自然科学基金项目(2022JJ30299、2022JJ30290)。

摘　　要：针对传统高果糖糖浆生产依赖的葡萄糖异构酶催化效率低等问题,本研究通过挖掘猕猴桃根部共生分散泛菌(Pantoea dispersa)的新型葡萄糖异构酶Pd-xylA基因,并实现其高效异源表达,将Pd-xylA基因克隆至大肠杆菌BL21(DE3)中进行表达,并对其诱导温度、IPTG(异丙基-β-D-硫代半乳糖苷)浓度等条件进行优化。结果表明,Pd-xylA最佳的诱导条件为诱导温度28℃、IPTG浓度0.1 mmol/L,在此条件下Pd-xylA可溶性表达量最高。本研究可以为开发具有更高催化活性与工业适用性的酶资源,以及生物法生产高果糖糖浆提供理论支持。To address the limitations of traditional high-fructose syrup production,such as low catalytic efficiency of conventional glucose isomerases,this study focused on the heterologous expression and optimization of a novel glucose isomerase,Pd-xylA,derived from Pantoea dispersa isolated from kiwifruit roots.The Pd-xylA gene was cloned and expressed in Escherichia coli BL21(DE3),followed by systematic optimization of induction conditions including temperature and IPTG(isopropyl-β-D-thiogalactopyranoside)concentration.The results demonstrated that the optimal induction conditions were 28℃ and 0.1 mmol/L IPTG,under which Pd-xylA achieved the highest soluble expression level.This study provided theoretical support for the development of enzyme resources with enhanced catalytic activity and industrial applicability,thereby advancing the biotechnological production of high-fructose syrup.

关 键 词：分散泛菌 葡萄糖异构酶 诱导条件优化 异源表达 

分 类 号：O561.3[理学—原子与分子物理]