胰腺导管腺癌组织中DLGAP5表达水平及其与患者预后的关系  

Correlation between DLGAP5 expression in pancreatic ductal adenocarcinoma tissue and patient prognosis

作　　者：张文姣 谢方瑜[3] 张巍巍[4] 付来琳 吕梅[4] 刘静依 郝明星 解祥军[4] ZHANG Wenjiao;XIE Fangyu;ZHANG Weiwei;FU Lailin;LYU Mei;LIU Jingyi;HAO Mingxing;XIE Xiangjun(Qingdao Medical College of Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学青岛医学院,山东青岛266071 [2]山东省公共卫生临床中心肝硬化一科 [3]青岛市市立医院心内科 [4]青岛市市立医院消化内科

出　　处：《精准医学杂志》2025年第2期117-120,126,共5页Journal of Precision Medicine

基　　金：青岛市民生科技计划项目(19-6-1-22-nsh)。

摘　　要：目的检测Discs大同源物关联蛋白5(DLGAP5)在胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDAC)患者肿瘤组织中的表达情况,并分析其与PDAC患者临床病理特征及预后的关系。方法选取2018年9月—2022年12月于青岛市市立医院行肿瘤切除的PDAC患者共75例,采用免疫组织化学(IHC)染色法检测DLGAP5蛋白在肿瘤组织及癌旁正常组织中的表达情况;根据DLGAP5表达水平将DLGAP5阳性患者分为DLGAP5低表达组和DLGAP5高表达组,分析其临床病理特征与DLGAP5表达水平的关系;通过多因素Cox回归模型分析DLGAP5表达阳性PDAC患者预后的相关危险因素;通过绘制Kaplan-Meier生存曲线分析DLGAP5表达水平与DLGAP5表达阳性PDAC患者生存率关系。结果IHC染色结果显示,DLGAP5在PDAC肿瘤组织中的表达水平显著高于癌旁正常组织(χ^(2)=36.930,P<0.05);临床资料分析结果显示,DLGAP5低、高表达组患者间的肿瘤分化程度、T分期、淋巴结转移及AJCC分期等临床病理特征差异有显著性(χ^(2)=6.222~10.940,P<0.05);多因素Cox回归模型分析结果显示,DLGAP5高表达是DLGAP5表达阳性的PDAC患者预后不良的独立危险因素(P<0.05);Kaplan-Meier生存曲线分析结果显示,DLGAP5高表达组患者的累积生存率显著低于DLGAP5低表达组(χ^(2)=18.929,P<0.05)。结论肿瘤分化程度低、T_(3)+T_(4)期、有淋巴结转移及Ⅱ期+Ⅲ期的PDAC患者肿瘤组织中DLGAP5高表达的概率显著升高,DLGAP5高表达与PDAC患者预后不良有关,DLGAP5有望成为PDAC诊断和预后分析的新型生物标志物。Objective To measure the expression of discs large-associated protein 5(DLGAP5)in pancreatic ductal adenocarcinoma(PDAC)tissue,and to analyze its correlation with clinicopathological features and patient prognosis.Methods A total of 75 patients with PDAC who underwent surgical resection in Qingdao Municipal Hospital from September 2018 to December 2022 were selected.Immunohistochemical(IHC)staining was used to measure the expression of DLGAP5 protein in tumor and adjacent normal tissues.Patients with positive DLGAP5 expression were divided into low and high DLGAP5 expression groups according to DLGAP5 expression levels.The correlation between clinicopathological features and DLGAP5 expression levels was analyzed.A multivariate Cox regression model was used to analyze the prognostic risk factors in patients with positive DLGAP5 expression.A Kaplan-Meier survival curve was plotted to analyze the relationship between DLGAP5 expression levels and survival rates in these patients.Results IHC staining showed significantly higher expression DLGAP5 levels in PDAC tumor tissues than in adjacent normal tissues(χ^(2)=36.930,P<0.05).Clinical data analysis showed significant differences between the low and high DLGAP5 expression groups in tumor differentiation,T stage,lymph node metastasis,and American Joint Committee on Cancer stage(χ^(2)=6.222-10.940,P<0.05).Multivariate Cox regression model analysis showed that high DLGAP5 expression was an independent risk factor for poor prognosis in patients with positive DLGAP5 expression.The Kaplan-Meier survival curve showed that the cumulative survival rate was significantly lower in patients with high DLGAP5 expression than in those with low expression(χ^(2)=18.929,P<0.05).Conclusion DLGAP5 is highly expressed in the tumor tissues of patients with poorly diffe-rentiated PDAC,stage T_(3)+T_(4)tumor,lymph node metastasis,and stageⅡ+Ⅲtumor.High DLGAP5 expression correlates with the poor prognosis in patients with PDAC.DLGAP5 shows promise as a new biomarker for PDAC diagnosis a

关 键 词：癌 胰腺管 腺癌 Discs大同源物关联蛋白5 危险因素 预后 

分 类 号：R735.9[医药卫生—肿瘤]