粉叶小檗根中非生物碱类化学成分研究  

Non-Alkaloid Chemical Constituents of the Roots of Berberis pruinosa

作　　者：冷玲 陈威 李港 姜北 肖朝江 Leng Ling;Chen Wei;Li Gang;Jiang Bei;Xiao Chaojiang(Yunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan,Dali,Yunnan 671000,China;Institute of Materia Medica,Dali University,Dali,Yunnan 671000,China;College of Pharmacy,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]云南省滇西抗病原植物资源筛选研究重点实验室,云南大理671000 [2]大理大学药物研究所,云南大理671000 [3]大理大学药学院,云南大理671000

出　　处：《大理大学学报》2025年第4期1-5,共5页Journal of Dali University

基　　金：国家自然科学基金项目(82460689);云南省中青年学术和技术带头人后备人才项目(202405AC350025);云南省专家工作站项目(202405AF140029);云南省滇西抗病原植物资源筛选研究重点实验室开放课题项目(APR2023Y06)。

摘　　要：目的:了解粉叶小檗Berberis pruinosa Franch.根的化学成分及其抗疟活性。方法:采用多种柱色谱分离手段,对粉叶小檗干燥根95%乙醇提取物的乙酸乙酯萃取部位的化学成分进行分离纯化,依据理化性质及现代波谱技术对化合物的结构进行分析鉴定,并对部分化合物进行抗疟活性筛选。结果:得到9个化合物,分别为:丁香酸(1)、3,4-二羟基苯甲酸甲酯(2)、4-羟基-3-甲氧基苯乙醇(3)、吐叶醇(4)、4-乙酰甲基-3,5-二甲氧基对醌醇(5)、pinellic acid(6)、辛二酸(7)、棕榈酸(8)和正二十九烷(9);活性测试结果显示,化合物5在50μmol/L浓度下具有较好的抗疟活性。结论:所有化合物均为首次从小檗属植物中分离得到。Objective:To investigate the chemical constituents of the roots of Berberis pruinosa,and their antimalarial activities.Methods:Chemical constituents were isolated and purified from the ethyl acetate fraction of the 95%ethanol extract of dried roots of B.pruinosa using various column chromatography techniques.The structures of the isolated compounds were elucidated based on their physicochemical properties and modern spectroscopic methods.Antimalarial activity of selected compounds was evaluated.Results:Nine compounds were identified:syringic acid(1),methyl 3,4-dihydroxybenzoate(2),4-hydroxy-3-methoxyphenylethanol(3),vomifoliol(4),4-acetylmethyl-3,5-dimethoxybenzoquinol(5),pinellic acid(6),suberic acid(7),palmitic acid(8),and nnonacosane(9).Activity assays showed that compound 5 exhibited significant antimalarial activity at a concentration of 50μmol/L.Conclusion:All compounds were isolated for the first time from Berberis species.

关 键 词：小檗属 化学成分 酚类 脂肪族类 结构鉴定 

分 类 号：R285[医药卫生—中药学]