双金属MOF纳米酶用于高效比色检测抗坏血酸  

Bimetallic MOF Nanozyme for Efficient Colorimetric Detection of Ascorbic Acid

作　　者：舒畅 桂凯欣 张颜 高胜寒 付雨欣 胡丁元 阎孟霞 李芳 SHU Chang;GUI Kaixin;ZHANG Yan;GAO Shenghan;FU Yuxin;HU Dingyuan;YAN Mengxia;LI Fang(Dezhou University,College of Chemistry and Chemical Engineering,Dezhou Shandong 253023,China;Dezhou University,College of Pharmacy,Dezhou Shandong 253023,China;Dezhou University,Institute of Biophysics,Dezhou Shandong 253023,China;Laoling People’s Hospital,Department of Laboratory,Dezhou Shandong 253600,China)

机构地区：[1]德州学院化学与化工学院,山东德州253023 [2]德州学院药学院,山东德州253023 [3]德州学院生物物理研究院,山东德州253023 [4]乐陵市人民医院检验科,山东德州253600

出　　处：《德州学院学报》2025年第2期78-82,共5页Journal of Dezhou University

基　　金：德州学院2024年国家级大学生创新创业训练计划项目(202410448008)。

摘　　要：利用Cu2+和Fe3+与单宁酸(TA)分子间的强配位结合,通过简单、绿色的方法合成了一种双金属有机框架(CuFeTAMOF)纳米酶。TA中的多酚具有还原性,可确保形成异价金属对(Fe3+/Fe2+、Cu2+/Cu+),金属对之间的自循环以及两种金属的协同调控作用使得该材料表现出优异的类氧化酶和类过氧化物酶催化活性。利用其对3,3’,5,5’-四甲基联苯胺(TMB)/过氧化氢显色体系的催化作用和抗坏血酸(AA)对显色反应的抑制效应,实现了对AA的高效检测,检测范围为3.33~146.7μmol/L,检测限为1.09μmol/L。Utilizing the strong coordination bonding between Cu2+and Fe3+and tannic acid(TA)molecules,a bimetallic organic framework(CuFeTAMOF)nanozyme was synthesized in a simple and green way.The polyphenols in TA have reducing properties,which ensures the formation of hetero-valent metal pairs(Fe3+/Fe2+,Cu2+/Cu+).The self-cycling between metal pairs and the synergistic reg ulation of the two metals make the material exhibit excellent oxidase-and peroxidase-like catalytic activities.Taking advantage of its catalytic effect on the 3,3’,5,5’-tetramethylbenzidine(TMB)/hydrogen peroxide chromogenic system and the inhibitory effect of ascorbic acid(AA)on the chromogenic reaction,a highly sensitive and selective detection of AA was achieved in the range of 3.33~146.7μmol/L,with a detection limit of 1.09μmol/L.

关 键 词：纳米酶 金属有机框架 比色传感 抗坏血酸 协同调控 

分 类 号：Q657[生物学—生物物理学]