呼气中痕量难电离一氧化氮质谱检测方法  

Mass spectrometry method for trace ionization-free detection of nitric oxide in exhaled breath

作　　者：何明晋 李斌[2] 苏蕊 朱腾高 杨彤彤 张文博 曾鹏 王晓芬 陈焕文 He Mingjin;Li Bin;Su Rui;Zhu Tenggao;Yang Tongtong;Zhang Wenbo;Zeng Peng;Wang Xiaofen;Chen Huanwen(Jiangxi Province Key Laboratory for Diagnosis,Treatment,and Rehabilitation of Cancer in Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Academician Workstation,Jiangxi University of Chinese Medicine,Nanchang 330004,China;State Key Laboratory of Inorganic Synthesis and Preparative Chemistry,College of Chemistry,Jilin University,Changchun 130012,China;Cancer Research Center,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学恶性肿瘤中医药诊疗与康复江西省重点实验室,南昌330004 [2]江西中医药大学院士工作站,南昌330004 [3]吉林大学化学学院无机合成与制备化学国家重点实验室,长春130012 [4]江西中医药大学癌症研究中心,南昌330004

出　　处：《分析仪器》2025年第2期54-59,共6页Analytical Instrumentation

基　　金：江西中医药大学校级科技创新团队发展计划(CXTD22005);江西中医药大学国家重大科研培育计划项目(2023ZDPY003);国家自然科学基金(22376084)。

摘　　要：本研究开发了一种新型非侵入性手持取样器,并结合电喷雾电离质谱(ESI-MS)技术,快速检测呼气中的一氧化氮(NO),以满足其在炎症检测及疗效评估中的需求。方法学验证结果显示,NO的线性范围为0.5~250μg/L,线性相关系数(R^(2))达到0.9960,检出限(LOD)为0.077μg/L,回收率在98.35%至105.34%之间,相对标准偏差(RSD)小于4.83%。通过对健康人群和尘肺患者的呼气样本进行检测,验证了该系统在复杂基质下的NO检测能力,并对不同人群的差异性进行了分析。This study developed a novel non-invasive handheld sampler,which could quickly detect nitric oxide(NO)in exhaled breath by combining with the electrospray ionization mass spectrometry(ESI-MS)technology to meet the needs of inflammation assessment and therapeutic evaluation.Methodological validation demonstrated that the linear detection range for NO was from 0.5 to 250μg/L,the high correlation coefficient(R^(2))was 0.9960.The limit of detection(LOD)was 0.077μg/L,and the recovery rates ranged from 98.35%to 105.34%,the relative standard deviation(RSD)was less than 4.83%.Analysis of exhaled breath samples from healthy individuals and pneumoconiosis patients confirmed the system's capability to detect NO in complex matrices and highlighted differences among populations.

关 键 词：电喷雾电离质谱(ESI-MS) 一氧化氮 呼气收集装置 尘肺患者 

分 类 号：R73[医药卫生—肿瘤]