基于GC-MS分析止咳桃花散中的挥发性成分  

Volatile Components in Zhike Taohua Powder by Gas Chromatography-Mass Spectrometry

作　　者：王媚 薛蕙 冯李乐 瞿钟欢 崔春利 张小飞 郭东艳 史亚军 WANG Mei;XUE Hui;FENG Lile;QU Zhonghuan;CUI Chunli;ZHANG Xiaofei;GUO Dongyan;SHI Yajun(Pharmacy College,Shaanxi University of Chinese Medicine/Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research,Xianyang 712046,China)

机构地区：[1]陕西中医药大学药学院/陕西省中药基础与新药研究重点实验室,陕西咸阳712046

出　　处：《中国现代中药》2025年第4期750-755,共6页Modern Chinese Medicine

基　　金：陕西省科技厅项目(2023-YBSF-474);陕西省中医药管理局中药制药工程重点学科资助项目(2017001);陕西省中药制药重点学科资助项目(303061107)。

摘　　要：目的:利用气相色谱-质谱法建立止咳桃花散的指纹图谱,并测定其中挥发性成分的含量,评价其质量,为后续改良止咳桃花散的制备工艺提供参考。方法:采用HP-5MS毛细管色谱柱;载气为氦气;流速为1.0 mL·min^(-1);分流比为25∶1;进样量为1.0μL;检测器温度为250℃;进样口温度为250℃;升温程序(初始温度82℃,保持20 min;10℃·min^(-1)至205℃,保持7 min;再以20℃·min^(-1)达到250℃)。结果:确定16个共有峰、11个特征峰,相似度均在0.99以上。其中,异龙脑、左旋龙脑、薄荷脑、麝香酮质量浓度分别为1.073 0~21.460 0、0.998 0~19.960 0、0.069 5~1.390 0、0.017 0~0.340 0μg·mL^(-1)(r=0.999 9)时线性关系良好。结论:建立的方法简单易行、灵敏度高、结果稳定可靠,可为止咳桃花散的质量评价提供参考。Objective:Gas chromatography-mass spectrometry(GC-MS)was used to establish a fingerprint of Zhike Taohua Powder and determine the content of volatile components to evaluate the quality of volatile components in Zhike Taohua Powder,providing a basis for evaluating the quality of volatile components in Zhike Taohua Powder and improving the preparation.Method:The analysis was performed on an HP-5MS capillary column,taking helium as the carrier gas at a flow rate of 1.0 mL·min^(-1) with a 25:1 split ratio and a 1.0μL injection volume.The detector was a mass spectrometer with a detector temperature of 250℃ and an inlet temperature of 250℃.The column temperature procedure was as follows:initial temperature at 82℃(held for 20 min),increased at 10℃·min^(-1) to 205℃(held for 7 min),then ramped at 20℃·min^(-1) to 250°C without holding.Results:There were sixteen common peaks and eleven core peaks,with similarities of more than 0.99.The concentration ranges of isoborneol,L-borneol,menthol,and muscone were 1.0730-21.4600,0.9980-19.9600,0.0695-1.3900,0.0170-0.3400μg·mL^(-1)(r=0.9999),respectively,with good linear relationships.Conclusion:The proposed method for determining volatile components in Zhike Taohua Powder is easy to implement with high sensitivity and reliable results,providing a reference for the quality evaluation of traditional Chinese medicine.

关 键 词：止咳桃花散 气相色谱-质谱法 挥发性成分 指纹图谱 含量测定 

分 类 号：R286[医药卫生—中药学]