牡丹籽饼粕提取物生物活性及活性成分分析  

Analysis of active ingredients and bioactivities of Paeonia suffruticosa Andr.seed cake extracts

作　　者：陈雨婷 尹佳欣 金银哲[2] 邹逸泓 金萍 陶俊 金英善[1,4] CHEN Yuting;YIN Jiaxin;JIN Yinzhe;ZOU Yihong;JIN Ping;TAO Jun;JIN Yingshan(College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;College of Horticulture and Landscape Architecture,Yangzhou University,Yangzhou 225009,China;Jiangsu Key Laboratory of Zoonosis,Yangzhou University,Yangzhou 2250o9,China)

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009 [2]上海海洋大学食品学院,上海201306 [3]扬州大学园艺园林学院,江苏扬州225009 [4]扬州大学江苏省人兽共患病学重点实验室,江苏扬州225009

出　　处：《扬州大学学报(农业与生命科学版)》2025年第1期110-120,共11页Journal of Yangzhou University:Agricultural and Life Science Edition

基　　金：国家重点研发计划项目(2019YFD1001502);江苏省人兽共患病重点实验室开放课题(R2109);江苏省研究生实践创新基金项目(SJCX21-1598)。

摘　　要：为有效利用牡丹籽饼粕资源,探究牡丹籽饼粕不同提取物体外抗氧化、抗胆碱酯酶和抑菌活性,利用液相色谱-质谱联用仪(LC-MS)分析牡丹籽饼粕活性成分,并利用分子对接技术分析其活性成分及潜在作用靶点蛋白。结果表明:牡丹籽饼粕70%甲醇提取物的总酚含量最高[(159.50±4.29)mg·g^(-1)],其体外抗氧化、抗胆碱酯酶和抑菌活性最强。LC-MS分析结果表明,牡丹籽饼粕70%甲醇提取物的主要活性成分为芍药苷、suffruticosol A、suffruticosol B、suffruticosol C和ε-viniferin。分子对接结果表明,牡丹籽饼粕活性成分与多种抗氧化和抗衰老靶点蛋白有较好的结合能力,其中ε-viniferin结合能力最强。综上,牡丹籽饼粕70%甲醇提取物的抗氧化、抗衰老和抑菌活性最高,这主要与多酚类的低聚芪类活性成分有关。In order to make effective use of Paeonia suffruticosa Andr.(peony)seed meal resources,the antioxidant activities,anticholinesterase activities and antibacterial activities of different extracts of peony seed meal were compared and studied in vitro.The active components of peony seed meal were analyzed by liquid chromatography mass spectrometry(LC-MS),and the active components and potential target proteins were analyzed by molecular docking technology.The results showed that the total phenol contents of 70%methanol extract of peony seed meal was the highest[(159.50±4.29)mg·g^(-1)],and its antioxidant,anticholinesterase and antibacterial activities were the strongest in vitro.LC-MS analysis showed that the main active components of 70%methanol extract of peony seed meal were paeoniflorin,suffruticosol A,suffruticosol B,suffruticosol C andε-viniferin.Molecular docking results showed that the active components of peony seed meal had good binding ability to various anti-oxidation and anti-aging target proteins,among whichε-viniferin had the strongest binding ability.The results indicated that the 70%methanol extract of peony seed meal had the highest antioxidant,anti-aging and antibacterial activities,which were mainly related to the active components of polyphenols oligostilbene.

关 键 词：牡丹籽饼粕 提取物 抗氧化活性 抗胆碱酯酶活性 抗菌活性 

分 类 号：S816.43[农业科学—饲料科学] TS229[农业科学—畜牧学]