A novel treatment approach using vitamin B12-conjugated sericin for mitigating nanodiamond-induced toxicity in darkling beetles  

作　　者：Marwa Saad Nabila Selim Lamia M.El-Samad 

机构地区：[1]Faculty of Science,Department of Zoology,Alexandria University,Baghdad st.,Qism Moharram Bek,Alexandria,Egypt

出　　处：《Insect Science》2025年第2期551-584,共34页昆虫科学(英文版)

摘　　要：The escalating use of nanodiamonds(NDs)has raised concerns about their ecotoxicological impact,prompting exploration of therapeutic interventions.This paper pioneers the examination of Vitamin B12-conjugated sericin(VB12-SER)as a potential therapeutic approach against ND-induced toxicity in darkling beetles(Blaps polychresta).The study analyzes mortality rates and organ-specific effects,covering the testis,ovary,and midgut,before and after treatments.Following exposure to 10 mg NDs/g body weight,within a subgroup of individuals termed ND2 with a mortality rate below 50%,two therapeutic treatments were administered,including pure sericin(SER)at 10 mg/mL and VB12-SER at 10.12 mg/mL.Consequently,five experimental groups(control,SER,ND2,ND2+SER,ND2+SER+VB12)were considered.Kaplan–Meier survival analysis was performed to assess the lifespan distribution of the insects in these groups over a 30-d period.Analyses revealed increased mortality and significant abnormalities induced by NDs within the examined organs,including cell death,DNA damage,enzyme dysregulation,antioxidant imbalances,protein depletion,lipid peroxidation,and morphological deformities.In contrast,the proposed treatments,especially(ND2+SER+VB12),demonstrated remarkable recovery,highlighting VB12-conjugated SER's potential in mitigating ND-triggered adverse effects.Molecular docking simulations affirmed binding stability and favorable interactions of the VB12-SER complex with target proteins.This research enhances understanding of NDs’effects on B.polychresta,proposing it as an effective bioindicator,and introduces VB12-conjugated SER as a promising therapeutic strategy in nanotoxicological studies.

关 键 词：Blaps polychresta molecular biochemical and microscopic analyses multiorgan assessment nanodiamond toxicity therapeutic interventions vitamin B12-conjugated sericin 

分 类 号：X171[环境科学与工程—环境科学]