基于生物信息学分析骨膜蛋白在胃癌中的临床意义及作用机制  

Clinical significance and mechanism of periostin in gastric cancer based on bioinformatics analysis

作　　者：陈雨濛 宣丽颖[1] 付卓 CHEN Yumeng;XUAN Liying;FU Zhuo(School of Basic Medicine Sciences,Inner Mongolia Minzu University,Tongliao,Inner Mongolia 028000,China;Kailu County Dongfeng Town Moral Health Center,Tongliao,Inner Mongolia 028406,China)

机构地区：[1]内蒙古民族大学基础医学院,内蒙古通辽028000 [2]开鲁县东风镇道德卫生院,内蒙古通辽028406

出　　处：《现代医药卫生》2025年第4期840-845,共6页Journal of Modern Medicine & Health

基　　金：内蒙古自治区自然科学基金项目(2020MS08031);内蒙古民族大学科研启动基金项目(KYQD23014)。

摘　　要：目的基于生物信息学方法分析骨膜蛋白(POSTN)在胃癌中的临床意义及作用机制。方法使用UALCAN数据库对POSTN基因在泛癌和胃癌中的表达情况进行分析,通过cBioPortal数据库对POSTN在泛癌和胃癌中的基因改变情况进行研究;利用Human Protein Atlas数据库对比POSTN基因编码蛋白在胃癌组织与非癌性胃组织表达的差异;通过Kaplan-Meier Plotter数据库获得POSTN表达水平与胃癌患者生存时间之间的关系;使用STRING数据库构建POSTN的相关蛋白网络。结果POSTN基因在泛癌中普遍表达;在胃癌组织中,POSTN基因和蛋白表达水平明显高于正常胃组织,差异有统计学意义(P<0.05);生存分析结果显示,POSTN高表达的胃癌患者,其生存时间明显短于POSTN低表达的患者,差异有统计学意义(P<0.05);此外,STRING数据库显示,与POSTN互作的蛋白主要包括FN1、BMP1、COL3A1、NOTCH1、COL1A2等,主要与胶原纤维构建、细胞外基质构建、血管发育、组织发育等过程相关。结论POSTN在胃癌中高度表达,并且与预后呈负相关,提示POSTN可能作为胃癌诊断与预后评估的新型分子标志物。Objective To analyze the clinical significance and mechanism of periostin(POSTN)in gastric cancer based on bioinformatics methods.Methods The expression of POSTN gene in pan-cancer and gastric cancer was analyzed by UALCAN database,and the gene changes of POSTN in pan-cancer and gastric cancer were studied by cBioPortal database.The Human Protein Atlas database was used to compare the differences in the expression of POSTN gene-encoded proteins in gastric cancer tissues and non-cancerous gastric tissues.The relationship between POSTN expression level and survival time of gastric cancer patients was obtained by Kaplan-Meier Plotter database.The STRING database was used to construct a network of POSTN-related proteins.Results POSTN gene was widely expressed in pan-cancer.The expression levels of POSTN gene and protein in gastric cancer tissues were significantly higher than those in normal gastric tissues(P<0.05).The results of survival analysis showed that the survival time of gastric cancer patients with high expression of POSTN was significantly shorter than that of patients with low expression of POSTN,and the difference was statistically significant(P<0.05).In addition,the STRING database showed that the proteins interacting with POSTN mainly include FN1,BMP1,COL3A1,NOTCH1,COL1A2,etc.,which are mainly related to collagen fiber construction,extracellular matrix construction,vascular development,tissue development and other processes.Conclusion POSTN is highly expressed in gastric cancer and negatively correlated with prognosis,suggesting that POSTN may be used as a new molecular marker for the diagnosis and prognosis of gastric cancer.

关 键 词：POSTN 胃癌 生物信息学 作用机制 

分 类 号：R735.2[医药卫生—肿瘤]