数字聚合酶链式反应在临床诊断中的应用研究进展  

Application research progress of digital polymerase chain reaction in clinical diagnosis

作　　者：陈伶行 陈琳[3] 周华[1,2] CHEN Lingxing;CHEN Lin;ZHOU Hua(Zhejiang University School of Medicine,Hangzhou,Zhejiang 310000,China;Department of Respiratory Medicine,The First Hospital Affiliated Zhejiang University School of Medicine,Hangzhou,Zhejiang 310000,China;Department of Infectious Diseases,Ningbo Second Hospital,Ningbo,Zhejiang 315000,China)

机构地区：[1]浙江大学医学院,浙江杭州310000 [2]浙江大学医学院附属第一医院呼吸内科,浙江杭州310000 [3]宁波市第二医院感染科,浙江宁波315000

出　　处：《现代医药卫生》2025年第4期1010-1014,共5页Journal of Modern Medicine & Health

摘　　要：数字聚合酶链式反应(dPCR)是继第一代普通聚合酶链式反应(PCR)和第二代荧光定量PCR(qPCR)之后的第三代核酸检测技术,具有检测周期短、灵敏度高等优势。dPCR既往研究主要应用在环境和农业相关检测。随着近些年新型冠状病毒感染疫情流行,越来越多研究关注dPCR在疾病诊断与预后方面的作用,此后该技术在感染性疾病、寄生虫疾病和肿瘤中均有研究。该文综述了dPCR在上述疾病诊断中的应用现状,以及与其他检测方法进行比较,同时总结dPCR在诊断疾病方面目前存在的问题及展望未来发展,供同行参考。Digital polymerase chain reaction(dPCR)is the third generation of nucleic acid detection technology following the first generation of conventional polymerase chain reaction(PCR)and the second generation of fluorescent quantitative PCR(qPCR).It boasts advantages such as a short detection cycle and high sensitivity.Previous research on dPCR was primarily focused on environmental and agricultural-related detections.With the prevalence of the COVID-19 pandemic in recent years,an increasing number of studies have highlighted the role of dPCR in disease diagnosis and prognosis.Since then,this technology has been studied in infectious diseases,parasitic diseases,and tumors.This article reviewed the current status of dPCR in the diagnosis of the aforementioned diseases and compared it with other detection methods.Additionally,it summarized the current issues with dPCR in disease diagnosis and outlines the future development prospects for reference by peers.

关 键 词：数字聚合酶链式反应 临床诊断 感染性疾病 肿瘤 遗传性疾病 综述 

分 类 号：R446[医药卫生—诊断学]