Hypoxia-inducible factor-1αat the invasive tumor front in oral squamous cell carcinoma  

作　　者：Felipe Martins Silveira Lauren Frenzel Schuch Vanesa Pereira-Prado Nelly Molina-Frechero Sandra Lopez-Verdin Marcelo Gómez Palacio-Gastélum Miguel Arocena Sven Niklander Estefania Sicco Ronell Bologna-Molina 

机构地区：[1]Molecular Pathology Area,Department of Diagnosis in Pathology and Oral Medicine,School of Dentistry,Universidad de la República,Montevideo 11600,Uruguay [2]Division of Biological and Health Sciences,Autonomous Metropolitan University,Coyoacan 04960,Mexico [3]Health Science Center,Research Institute of Dentistry,Universidad de Guadalajara,Guadalajara 44100,Jalisco,Mexico [4]Department of Research,Universidad Juarez del Estado de Durango,Durango 34000,Mexico [5]Departamento de Biología Odontológica,Facultad de Odontología,Universidad de la República,Montevideo 11600,Uruguay [6]Unit of Oral Pathology and Medicine,Faculty of Dentistry,Universidad Andres Bello,Viña del Mar 2520000,Chile

出　　处：《World Journal of Experimental Medicine》2025年第2期83-92,共10页世界实验医学杂志(英文)

基　　金：Supported by Comisión Sectorial de Investigación Científica(CSIC-Research Group 88180);The Agencia Nacional de Investigación e Innovación/Sistema Nacional de Investigadores(ANII/SNI);The Programa de Desarrollo de las Ciencias Básicas(PEDECIBA),Uruguay.

摘　　要：BACKGROUND Hypoxia in oral cancer promotes tumoral invasion by inducing epithelial-mesenchymal transition,leading to aggressive tumor progression.AIM To characterize the expression of hypoxia-inducible factor 1-alpha(HIF-1α)at the invasive tumor front(ITF)in comparison to tumor islands(TI)in oral squamous cell carcinoma(OSCC)and to explore its relationship with E-cadherin and Vimentin expression.METHODS Thirty-eight cases of OSCC and five cases of normal oral mucosa(NOM)were included in this study.The ITF was identified based on the region and immune expression of AE1/AE3.Immunohistochemistry was performed to assess the expression of HIF-1α,Vimentin,and E-cadherin.The immunostaining was analyzed using an immunoreactive score,and the results were illustrated using immunofluorescence.RESULTS HIF-1αexpression was significantly higher in the TI region compared to the ITF region(P=0.0134).Additionally,a significant difference was observed between TI and NOM(P=0.0115).In the ITF regions,HIF-1αexpression showed a significant correlation with Vimentin expression,with higher levels of HIF-1αassociated with increased Vimentin expression(P=0.017).CONCLUSION Based on the results of this study,HIF-1αappears to play a distinct role in OSCC tumor progression,underscoring the importance of exploring hypoxia-driven changes in cellular phenotype at the ITF of OSCC.Further research is needed to better understand their impact on OSCC prognosis.

关 键 词：Hypoxia-inducible factor-1α Oral squamous cell carcinoma IMMUNOHISTOCHEMISTRY VIMENTIN E-CADHERIN 

分 类 号：R739.8[医药卫生—肿瘤]