Anti-hypertensive effect of enriched white melon seed protein concentrate biscuit on sodium fluoride exposed rats  

作　　者：Olamide Wilson Fasakin Ayoola Awosika Sanmi Tunde Ogunsanya Iyanuoluwa Olushola Benson Akinyode Isaac Olopoda 

机构地区：[1]Department of Biomedical Technology,Federal University of Technology,Akure 234034,Nigeria [2]College of Medicine,University of Illinois,Chicago,Peoria,IL 61601,United States [3]Department of Anatomy,Babcock University,Ilishan-Remo 23401,Ogun State,Nigeria [4]Department of Anatomy,Osun State University,Oshogbo 234030,Osun,Nigeria [5]Department of Biochemistry,Federal University of Technology,Akure 234034,Ondo,Nigeria

出　　处：《World Journal of Experimental Medicine》2025年第2期185-199,共15页世界实验医学杂志(英文)

摘　　要：Sodium fluoride(NaF)is a daily necessity consumed as the major ingredient of fluorinated drinking water,milk,salts,mouthwashes,toothpaste,and dentistry medications.However,the use of NaF products has also been associated with increased fluoride anion distribution in the body,leading to hypertension.AIM This study evaluated the antihypertensive effect of sweet orange peels-enriched white melon seed protein concentrate(WSP)biscuit meal in eight-week-old albino rats exposed to NaF for 14 days.METHODS Forty-two(42)male Wistar albino rats were assigned at random into 7 groups of 6 rats per group(control group and six experimental groups).The experimental groups received various treatments that lasted for two weeks.Twenty-four hours after the last administration,hemodynamic parameters were evaluated,rats were sacrificed,blood samples were collected,and the heart was harvested.Blood serum was assessed for cardiac troponin I(cTnI),creatine kinase-MB(CK-MB),and lactate dehydrogenase(LDH).At the same time,the heart homogenate was assayed for angiotensin-1 converting enzyme(ACE)activity,proinflammatory cytokines,nitric oxide concentrations,and antioxidant status.Cardiac tissues were stained with Hematoxylin and Eosin,Masson’s Trichrome,and cTnI.Also,the safety of the WSP biscuit diet was evaluated.RESULTS Results obtained showed that NaF administration elevated the collagen content of cardiac tissues,activities of ACE,and concentrations of cTnI,CK-MB,LDH,tumor necrosis factor-alpha,and interleukin 1 beta,while there was a reduction in the concentration of nitric oxide and antioxidants;however,their alterations were significantly prevented in WSP-biscuit-fed rats.The WSP biscuit meal is safe for consumption and possesses dose-dependent antihypertensive ability at 10%and 20%inclusion.CONCLUSION The WSP biscuit diet may be recommended in diet formulation for the management of individuals or communities that are predisposed to NaF contaminations.

关 键 词：HYPERTENSION Sodium fluoride Sweet orange peels White melon seed Protein concentrate 

分 类 号：R151.3[医药卫生—营养与食品卫生学]