TRIM24蛋白在人肾透明细胞癌组织中的表达及其临床意义  

Expression of TRIM24 protein in human clear cell renal cell carcinoma tissue and its clinical significance

作　　者：耿海营 于焱 代春美 温有锋 李宁 GENG Haiying;YU Yan;DAI Chunmei;WEN Youfeng;LI Ning(Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Jinzhou Medical University,Jinzhou 121000,China;Department of Pathology and Pathophysiology,Shenyang Medical College,Shenyang 110000,China;Institute of Drug Research,School of Life Sciences,Jinzhou Medical University,Jinzhou 121000,China;Biological Anthropology Institute,School of Life Sciences,Jinzhou Medical University,Jinzhou 121000,China;Key Laboratory of Human Ethnic Specificity and Phenomics of Critical Illness in Liaoning Province,Shenyang Medical College,Shenyang 110000,China;Key Laboratory of Phenomics in Shenyang City,Shenyang Medical College,Shenyang 110000,China;Department of Biochemistry and Molecular Biology,Shenyang Medical College,Shenyang 110000,China)

机构地区：[1]锦州医科大学基础医学院生物化学与分子生物学教研室,辽宁锦州121000 [2]沈阳医学院病理学与病理生理学教研室,辽宁沈阳110000 [3]锦州医科大学生命科学院药物研究所,辽宁锦州121000 [4]锦州医科大学生命科学院生物人类学研究所,辽宁锦州121000 [5]沈阳医学院辽宁省人类族群特异性及重要疾病表型组学重点实验室,辽宁沈阳110000 [6]沈阳医学院沈阳市表型组学研究重点实验室,辽宁沈阳110000 [7]沈阳医学院生物化学与分子生物学教研室,辽宁沈阳110000

出　　处：《吉林大学学报(医学版)》2025年第2期486-492,共7页Journal of Jilin University:Medicine Edition

基　　金：辽宁省教育厅科研面上项目(LJKZ0811,JYTMS20231393);辽宁省中央引导地方科技发展专项项目(2023JH6/100100021)。

摘　　要：目的:探讨三重基序蛋白24(TRIM24)在肾透明细胞癌(ccRCC)组织中的表达,阐明TRIM24蛋白与ccRCC患者临床病理特征和预后的关联。方法:选取术前均未行放化疗的90例ccRCC患者的癌组织和癌旁正常组织。采用组织芯片技术和免疫组织化学法检测ccRCC组织中TRIM24蛋白表达水平,分析ccRCC组织与癌旁正常组织中TRIM24蛋白表达水平的差异。计算TRIM24蛋白表达评分及其平均值,并根据平均值将ccRCC患者分为TRIM24蛋白低表达组和TRIM24蛋白高表达组;分析TRIM24蛋白表达水平与患者不同临床病理特征之间的关联,分析TRIM24蛋白表达水平与患者预后之间的关系。结果:免疫组织化学法,TRIM24蛋白在ccRCC患者癌组织细胞核和细胞质中均有表达,且癌组织中TRIM24蛋白表达水平与癌旁正常组织比较差异均有统计学意义(P<0.05);ccRCC组织细胞核中TRIM24蛋白表达水平与患者年龄、性别和肿瘤大小有关联(P<0.05);KaplanMeier生存分析,TRIM24蛋白在ccRCC组织细胞质中高表达、年龄大和病理分级高的患者总生存期短于TRIM24蛋白低表达、年龄小和病理分级低的患者(P<0.05);多因素Cox回归分析,与TRIM24蛋白低表达和病理分级低的患者比较,ccRCC组织细胞质中TRIM24蛋白高表达和病理分级高的患者预后较差(P<0.05)。结论:ccRCC癌组织的细胞质中TRIM24高表达和病理分级高的患者术后生存率较低,预后较差。Objective:To discuss the expression of tripartite motif-containing protein 24(TRIM24)in the clear cell renal cell carcinoma(ccRCC)tissue,and to clarify its relationships with the clinicopathological features and prognosis of the ccRCC patients.Methods:The cancer and paracancer normal tissues were collected from 90 ccRCC patients who had not undergone preoperative radiotherapy or chemotherapy.Tissue microarray and immunohistochemistry were used to detect the expression levels of TRIM24 protein in ccRCC tissue.The differences in TRIM24 protein expression between ccRCC and paracancer normal tissues were analyzed.The score of TRIM24 protein expression and the average value were calculated,and based on the average value,the patients were classified into TRIM24 protein low-expression and TRIM24 protein high-expression groups.The associations between the TRIM24 protein expression and different clinicopathological features of the patients were analyzed,and the relationship between the TRIM24 protein expression and the prognosis of the patients was analyzed.Results:The immunohistochemistry results showed that the TRIM24 protein was expressed in both the nucleus and cytoplasm of the ccRCC tumor cells,and there were significant differences in the TRIM24 protein expression level in ccRCC tissue when compared with paracancer normal tissue(P<0.05).The TRIM24 protein expression in the nucleus of ccRCC tissue was associated with the patient’s age,gender,and tumor size(P<0.05).The Kaplan-Meier survival analysis results showed that the overall survivals of the patients with high TRIM24 protein expression in the cytoplasm of ccRCC tissue,older age,and higher pathological grade were shorter than those with low TRIM24 protein expression,younger age,and lower pathological grade(P<0.05).The multivariate Cox regression analysis results showed that the prognosis of the patients with high TRIM24 protein expression in the cytoplasm and higher pathological grade were poorer compared with the patients with low TRIM24 protein expression and

关 键 词：肾透明细胞癌 三重基序蛋白24 免疫组织化学 病理分级 预后 

分 类 号：R737.11[医药卫生—肿瘤]