中国结核分枝杆菌微量肉汤稀释法药物敏感性试验标准化专家共识  

Expert consensus on the standardization of broth microdilution method for drug susceptibility testing of Mycobacterium tuberculosis in China

作　　者：中国防痨协会结核病基础专业分会 余方友 赵雁林[3] 杨景卉 夏辉[3] 黄海荣[4] 郝晓晖 江渊[5] 谭耀驹[6] Tuberculosis Basic Professional Branch,Chinese Antituberculosis Association;Yu Fangyou;Zhao Yanlin

机构地区：[1]不详 [2]同济大学附属上海市肺科医院 [3]中国疾病预防控制中心 [4]首都医科大学附属北京胸科医院 [5]上海市疾病预防控制中心 [6]广州市胸科医院

出　　处：《中国防痨杂志》2025年第5期535-545,共11页Chinese Journal of Antituberculosis

基　　金：上海市加强公共卫生体系建设三年行动计划(2023—2025年)(GWVI-11.1-05,GWVI-11.2-YQ52);上海市临床重点专科建设项目(结核病科)(shslezdzk03001);结核病专病数据库(SHDC2020CR6024)。

摘　　要：耐药结核病长期以来一直是全球公共卫生领域的严峻挑战,目前仅依赖二分类表型药物敏感性试验(简称“表型药敏试验”)和基因型药敏试验难以为患者提供精准有效的治疗方案。基于微孔板的微量肉汤稀释法药敏试验不仅能够一次性涵盖多种药物和多个浓度,还可以为临床提供量化的耐药信息,因此,受到越来越多的关注。为了合理、科学、规范地使用微量肉汤稀释法药敏试验,中国防痨协会结核病基础专业分会组织多位相关领域专家,针对该药敏方法的技术特点、应用价值、标准化操作、结果解读,以及实际操作中的注意事项等问题制定了本共识,并提出了12条推荐意见,旨在为结核病防治相关临床及实验室工作人员提供统一的指导原则。Drug resistant tuberculosis has long been a serious challenge in the field of global public health.At present,it is difficult to provide accurate and effective treatment plans for patients based solely on binary phenotypic drug sensitivity and genotypic drug sensitivity tests.The microbroth dilution drug sensitivity test based on the microplate not only covers a variety of drugs and multiple concentrations at one time,but also provides quantitative drug resistance information for the clinic,so it is receiving more and more attention.In order to use the microbroth dilution drug sensitivity test in a reasonable,scientific,and standardized manner,the Tuberculosis Basic Professional Branch of Chinese Antituberculosis Association had organized experts in relevant fields to formulate this consensus on technical characteristics,application value,standardized operation,result interpretation,and precautions in actual operation of this drug sensitivity method,and proposed 12 recommendations,aiming to provide unified guiding principles for clinical and laboratory staff who are related to tuberculosis.

关 键 词：分枝杆菌 结核 微生物敏感性试验 微量肉汤稀释法 总结性报告(主题) 

分 类 号：R-331[医药卫生] R372