盐度对Alcaligenes faecalis DN25降解氰化物的影响  

Effects of salinity on cyanide degradation by Alcaligenes faecalis DN25

作　　者：沈慧婷 李洪慧 李梅 方艺燕 李青云[1] SHEN Huiting;LI Honghui;LI Mei;FANG Yiyan;LI Qingyun(School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《生物加工过程》2025年第2期147-152,共6页Chinese Journal of Bioprocess Engineering

基　　金：广西大学科研基金(XJZ130360);广西高等教育本科教学改革工程项目(2021JGA111);广西大学生创新创业训练计划(S202210593363)。

摘　　要：废水中的盐度是影响生物降解活性的重要因素。为了进一步考察1株氰降解菌粪产碱杆菌(Alcaligenes faecalis)DN25的实际应用潜力,本实验开展了盐度对A.faecalis DN25降解氰化物的影响研究。结果表明:当盐度增大,菌株降解氰化物的时间变长;当盐度为8%(质量分数)时,100 mg/L氰化物的降解时间从无盐条件下的30 min延长至84 h;外源添加相容性溶质谷氨酸可提高氰化物降解率,在4%盐度下添加300 mg/L谷氨酸,菌株30 min可将100 mg/L的氰化物完全降解,而不添加谷氨酸的样品30 min的降解率为86.2%±0.9%,初步研究发现,谷氨酸可以快速进入细胞体内平衡渗透压,维持细胞的降解活性。可见,菌株Alcaligenes faecalis DN25在含盐废水处理中具有良好的应用潜力。The salinity of wastewater is an important factor to affect the biodegradation activity.To further evaluate the practical application potential of Alcaligenes faecalis DN25,the cyanide degradation of this strain was studied under different salinity levels.As a result,the cyanide degradation required much longer time with the increase of salinity.For example,the degradation time of 100 mg/L cyanide increased from 30 minutes to 84 hours when the salinity rose from 0 to 8%.Interestingly,the addition of glutamate,as a compatible solute,could significantly accelerate the cyanide degradation by strain DN25.Notably,under a salinity level of 4%,the addition of 300 mg/L glutamate could lead to complete degradation of 100 mg/L cyanide within just 30 minutes,whereas the degradation efficiency of the same sample without glutamate dropped to 86.2%±0.9%.Our preliminary studies revealed that glutamate could rapidly enter the cells to maintain osmotic pressure balance,thus sustaining the degradation activity.Overall,Alcaligenes faecalis DN25 exhibited promising potential for practical applications in the field of wastewater treatment.

关 键 词：盐度 谷氨酸 相容性溶质 氰化物 生物降解 Alcaligenes faecalis 

分 类 号：Q93[生物学—微生物学] X172[环境科学与工程—环境科学]