异源蛋白在枯草芽孢杆菌中高效表达策略研究进展  

Recent progress on efficient expression strategies of heterologous proteins in Bacillus subtilis

作　　者：聂珂珂 刘英[2,3] 王仕楷[1] 彭小伟 NIE Keke;LIU Ying;WANG Shikai;PENG Xiaowei(College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,China;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;Key Laboratory of Biopharmaceutical Preparation and Delivery,Chinese Academy of Sciences,Beijing 100190,China)

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [3]生物药制备与递送中国科学院重点实验室,北京100190

出　　处：《生物加工过程》2025年第2期212-225,共14页Chinese Journal of Bioprocess Engineering

基　　金：国家重点研发计划(2021YFC2101602);国家自然科学基金(22178362)。

摘　　要：枯草芽孢杆菌(Bacillus subtilis)具有遗传背景清晰、无密码子偏好、生长迅速、蛋白分泌能力强以及无致病性等优点,是异源蛋白表达的优选模式菌株。目前已有多种工业酶实现了其在枯草芽孢杆菌中的高效表达,如α淀粉酶、β葡聚糖酶、角蛋白酶和纳豆激酶等。本文从异源蛋白高效表达策略、异源蛋白分泌途径优化策略、菌株改造策略及发酵优化策略4个方面进行综述,系统地对枯草芽孢杆菌异源蛋白表达系统的优化策略进行归纳总结,并对如何提高异源蛋白产量提出了新的建议和策略。Bacillus subtilis is the optimal model strain for heterologous protein expression due to its advantages of clear genetic background,no codon preference,rapid growth,strong protein secretion ability and non-pathogenicity.At present,many industrial enzymes can be highly expressed in Bacillus subtilis,such asα-amylase,β-glucosidase,keratinase,nattokinase,etc.Herein,we summarized various strategies with regards to the expression and secretion pathway optimization for heterologous proteins,as well as the related strain modification and fermentation optimization.Based on our systematic review on heterologous protein expression system of Bacillus subtilis,we provided our outlook how to further improve the industrial production of heterologous proteins.

关 键 词：枯草芽孢杆菌 异源蛋白 高效表达策略 酶制剂 工业化应用 

分 类 号：Q789[生物学—分子生物学] TQ925[轻工技术与工程—发酵工程]