基于UPLC-Q Exactive MS的准噶尔柳花化学成分分析及抗脂肪肝作用体外机制研究  

UPLC-Q Exactive MS-Based analysis of chemical constituents in Salix songarica flowers and in vitro study of its mechanism against fatty liver

作　　者：余璐 董静茹 王兰兰 李好 戴雪梅 郭新勇[2] 李静 张丽[1] YU Lu;DONG Jing-ru;WANG Lan-lan;LI Hao;DAI Xue-mei;GUO Xin-yong;LI Jing;ZHANG Li(School of Medicine,Shihezi University;School of Life Sciences,Shihezi University,Shihezi 832003,China;Wenquan County Center for Disease Control and Prevention,Wenquan 833500,China)

机构地区：[1]石河子大学医学院 [2]石河子大学生命科学学院,新疆832003 [3]温泉县疾病预防控制中心,新疆833500

出　　处：《天然产物研究与开发》2025年第4期647-658,共12页Natural Product Research and Development

基　　金：国家自然科学基金(32160071);石河子大学科研计划项目青年拔尖计划项目(BJZK202404);师市级第七师胡杨河市财政科技计划(2023C19)。

摘　　要：探究准噶尔柳花的活性成分及其改善代谢功能障碍相关脂肪性肝病(metabolic dysfunction-associated steatotic liver disease,MASLD)的作用机制。研究采用超高效液相色谱-四极杆-静电场轨道阱质谱(UPLC-Q Exactive MS)技术分析了准噶尔柳花中的主要化学成分,共鉴定出36个化合物及其含量,涵盖黄酮类、多酚类、酸类及其他生物活性成分,其中木犀草苷、儿茶素、木犀草素等生物活性成分的含量较为丰富。通过游离脂肪酸(free fatty acids,FFA)诱导的HepG2细胞脂肪堆积模型,研究了准噶尔柳花提取物(Salix songarica flower extract,SSE)对MASLD的防治效果。实验结果显示,SSE显著地降低了细胞内的总胆固醇(total cholesterol,TC)和甘油三酯(triglyceride,TG)含量,减少了脂滴积累,并改善了肝损伤标志物天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)水平。同时,SSE还显著地降低了氧化损伤标志物丙二醛(malondialdehyde,MDA)的含量,提高了超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和还原型谷胱甘肽(reduced glutathione,GSH)水平。机制研究进一步揭示,SSE通过上调法尼醇X受体(farnesoid X receptor,FXR)、胆盐输出泵(bile salt export pump,BSEP)和胆固醇7α-羟化酶(cholesterol 7α-hydroxylase,CYP7A1)的mRNA表达水平,调节了肝细胞脂质代谢。综上,SSE在MASLD防治中展现了显著潜力,具备抗氧化和调节脂质代谢的功能,提供了新的预防与治疗MASLD的可能性,尤其在食品和保健品领域的应用前景广阔。This study aims to investigate the active components of Salix songarica flowers and their therapeutic mechanisms in mitigating metabolic dysfunction-associated steatotic liver disease(MASLD).The composition of S.songarica flower was analyzed using UPLC-Q Exactive MS,leading to the identification of 36 chemical compounds,including flavonoids,polyphenols,acids,and other biologically active components.Among these,luteoloside,catechins,and luteolin were found to be particularly abundant in terms of biologically active content.The efficacy of S.songarica flower extract(SSE)in combating MASLD was evaluated using a free fatty acid(FFA)-induced fat accumulation model in HepG2 cells.SSE significantly reduced intracellular levels of total cholesterol(TC)and triglycerides(TG),decreased lipid droplet formation,and improved liver injury markers,including aspartate aminotransferase(AST)and alanine aminotransferase(ALT).Additionally,SSE markedly lowered malondialdehyde(MDA)levels,a marker of oxidative damage,while increasing the activity of antioxidant enzymes,including superoxide dismutase(SOD),catalase(CAT),and reduced glutathione(GSH).Mechanistic studies further reveal that SSE regulates hepatic lipid metabolism by upregulating the mRNA expression levels of farnesoid X receptor(FXR),bile salt export pump(BSEP),and cholesterol 7α-hydroxylase(CYP7A1).In conclusion,SSE demonstrates significant potential for the prevention and treatment of MASLD,possessing both antioxidant and lipid metabolism-modulating properties.These findings open new avenues for the development of strategies to prevent and treat MASLD,particularly within the fields of food science and nutraceuticals.

关 键 词：脂肪肝 植物提取物 准噶尔柳花 UPLC-Q Exactive MS 

分 类 号：R151.3[医药卫生—营养与食品卫生学]