井冈山地区泥炭藓来源共生真菌Penicillium onobense JW13-1混源萜类代谢产物研究  

Meroterpenoids metabolites of symbiotic fungus Penicillium onobense JW13-1 from Sphagnum palustre L.in Jinggangshan

作　　者：叶强 赖健福 刘文杰 郭文强 YE Qiang;LAI Jian-fu;LIU Wen-jie;GUO Wen-qiang(School of Traditional Chinese Medicine;Jiangxi Province Key Laboratory of Organ Development and Epigenetics,Department of Medicine,Jinggangshan University,Ji′an 343009,China)

机构地区：[1]井冈山大学中医药学院 [2]井冈山大学医学部器官发育与表观遗传江西省重点实验室,吉安343009

出　　处：《天然产物研究与开发》2025年第4期667-673,共7页Natural Product Research and Development

基　　金：国家自然科学基金地区科学基金(82260690);江西省自然科学基金面上项目(20224BAB206120);吉安市科技局重点科技计划(20222-201741x)。

摘　　要：利用单菌多次级代谢产物策略对井冈山地区泥炭藓来源共生真菌Penicillium onobense JW-13-1次级代谢产物进行分子多样性探究。运用硅胶柱色谱、葡聚糖凝胶柱色谱和高效液相色谱等方法对该菌株静置条件的发酵产物进行分离纯化;通过核磁共振、质谱等方法鉴定化合物的分子结构;并对天然产物进行抗菌活性的初步评价。从该菌株的发酵粗提物中分离得到8个混源萜类天然产物,即asperanstinoid D(1)、dehydroaustin(2)、austin(3)、acetoxydehydroaustin(4)、austinol(5)、austinolide(6)、11β-acetoxyisoaustinone(7)和isoaustinol(8)。抗菌活性结果显示化合物1~3对金黄色葡萄球菌具有一定的抑制活性,说明该菌株具有产生抗菌活性天然产物的潜力。This study aims to investigate the molecular diversity of secondary metabolites of symbiotic fungus Penicillium onobense JW13-1 from Sphagnum palustre L.in Jinggangshan by using one strain many compounds strategy.The fermentation products were isolated and purified by silica gel column chromatography,sephadex column chromatography and high performance liquid chromatography.The chemical structures of compounds were identified by NMR and ESI-MS,which conducted preliminary evaluation of the antibacterial activity.Eight meroterpenoids were obtained and their structures were elucidated as asperanstinoid D(1),dehydroaustinn(2),austin(3),acetoxydehydroaustin(4),austinol(5),austinolide(6),11β-acetoxyisoaustinone(7),and isoaustinol(8).The antibacterial activity results showed that compounds 1-3 had certain inhibitory activity against staphylococcus aureus,indicating that the strain had the potential to produce natural products with antibacterial activity.

关 键 词：井冈山地区 泥炭藓 共生真菌 混源萜 抗菌活性 

分 类 号：R914.4[医药卫生—药物化学]