虾青素对斑马鱼高温胁迫损伤的缓解效应  

Astaxanthin mitigated oxidative damage to zebrafish under high-temperature stress

作　　者：梁书来 柳文燕 黄思倩 王永红 LIANG Shulai;LIU Wenyan;HUANG Siqian;WANG Yonghong(School of Food and Biological Engineering,Bengbu University,Bengbu 233030,China)

机构地区：[1]蚌埠学院食品与生物工程学院,安徽蚌埠233030

出　　处：《河北渔业》2025年第4期8-11,共4页

基　　金：安徽省优秀青年教师培育项目(YQYB2023056);校企合作项目“拟穴青蟹苗种繁育与人工养殖关键技术研究”(00014166);国家级大学生创新创业训练计划项目(202311305033);省级大学生创新创业训练计划项目(S202311305098)。

摘　　要：为探讨虾青素对斑马鱼(Danio rerio)高温胁迫损伤的缓解效应,给体质量为(0.17±0.03)g的斑马鱼投喂含3%虾青素的饲料42 d后,再将其暴露在水温为32℃的水体中24 h,测量其肝脏中丙二醛(MDA)、抗氧化指标[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)活性,以及谷胱甘肽(GSH)含量]和非特异性免疫指标[溶菌酶(LZM)和碱性磷酸酶(AKP)活性]。结果显示,高温胁迫增加了MDA含量,表明高温胁迫对机体造成了氧化损伤。虾青素降低了高温胁迫下斑马鱼的MDA含量,增加了高温胁迫下斑马鱼的SOD、CAT、GPx、LZM和AKP活性,以及GSH含量,表明虾青素可以通过提高机体的抗氧化性能和非特异性免疫力来缓解高温胁迫对斑马鱼的氧化损伤。To investigate the mitigative effects of astaxanthin on oxidative damage caused by high-temperature stress in zebrafish(Danio rerio),fish with an average body weight of(0.17±0.03)g was fed with a feed containing 3%astaxanthin for 42 days,and then exposed to high temperature(32℃)for 24 hours.Malondialdehyde(MDA),antioxidant indicators[superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx)activities,and glutathione(GSH)content],and non-specific immune indicators[lysozyme(LZM)and alkaline phosphatase(AKP)activities]in the liver of fish were analyzed.The results showed that high-temperature stress enhanced MDA content,suggesting that high-temperature stress induced oxidative damage to fish.Astaxanthin reduced MDA content,and increased SOD,CAT,GPx,LZM,and AKP activities,and GSH content when zebrafish was under high-temperature stress,indicating that astaxanthin inhibited oxidative damage by enhancing antioxidant performance and non-specific immunity.

关 键 词：虾青素 斑马鱼(Danio rerio) 高温胁迫 抗氧化性能 免疫 

分 类 号：S969.1[农业科学—水产养殖] D912[农业科学—水产科学]