Extramural venous invasion in gastric cancer: 9.4T magnetic resonance imaging assessment and circular RNA functional analysis  

作　　者：Cai-Zhen Feng Xin-Yi Gou Yi-Qun Liu Yu-Wei Xin Yin-Li Zhang Hui-Min Zhao Sheng-Cai Wei Nan Hong Yi Wang Jin Cheng 

机构地区：[1]Department of Radiology,Peking University People’s Hospital,Beijing 100044,China [2]Department of Ultrasound,Peking University People’s Hospital,Beijing 100044,China [3]Department of Pathology,Peking University People’s Hospital,Beijing 100044,China

出　　处：《World Journal of Gastroenterology》2025年第14期94-105,共12页世界胃肠病学杂志(英文)

基　　金：Supported by The National Natural Science Foundation of China,No.81901819;Peking University People’s Hospital Scientific Research Development Funds,No.RDX2024-08;Beijing Natural Science Foundation,No.7232187.

摘　　要：BACKGROUND Extramural venous invasion(EMVI)is a critical prognostic factor in gastric cancer(GC);however,its detection and underlying molecular mechanisms remain underexplored.AIM To investigate the relationship between EMVI and expression of the circular RNA hsa_circ_0097977 in orthotopic GC mouse models.METHODS A retrospective analysis was conducted in addition to a preclinical animal study,involving 13 GC patients and 24 orthotopic GC mouse models,respectively.EMVI was assessed using axial T2-weighted fat suppression sequences on a 9.4T magnetic resonance imaging(MRI)with histopathological confirmation as the gold standard for EMVI.The impact of hsa_circ_0097977 on EMVI and GC cell function was evaluated.Statistical analyses comprised consistency,area under the curve analysis,correlation,χ^(2)/Fisher exact,and Mann-Whitney U/t-tests,with significance set at P<0.05.RESULTS EMVI was accurately detected using 9.4T MRI in orthotopic mouse models with an area under the curve of 0.843(sensitivity 78.6%,specificity 90.0%).MRI detected EMVI was the only imaging factor associated with distant metastasis(P=0.04).Furthermore,knockdown of hsa_circ_0097977 was the only factor associated with EMVI(P=0.043,0.038)and led to reduced invasion and increased apoptosis in GC cells.CONCLUSION EMVI,a risk factor for distant metastasis in GC,is detectable by 9.4T MRI and regulated by hsa_circ_0097977,making it a potential therapeutic target.

关 键 词：Gastric cancer Circular RNA Extramural venous invasion Orthotopic mouse model Magnetic resonance imaging Pathology Radiogenomics 

分 类 号：R735.2[医药卫生—肿瘤]