Strain-and sex-dependent variability in hepatic microcirculation and liver function in mice  

作　　者：Bing Wang Yuan Li Qin Ouyang Meng-Ting Xu Ying-Yu Wang Sun-Jing Fu Wei-Qi Liu Xue-Ting Liu Hao Ling Xu Zhang Rui-Juan Xiu Ming-Ming Liu 

机构地区：[1]Institute of Microcirculation,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100005,China [2]International Center of Microvascular Medicine,Chinese Academy of Medical Sciences,Beijing 100005,China [3]Department of Pathology,Wangjing Hospital,China Academy of Chinese Medical Science,Beijing 100102,China [4]Department of Cardiology,Peking University China-Japan Friendship School of Clinical Medicine,Beijing 100029,China [5]Department of Radiology,The Affiliated Changsha Central Hospital,Hengyang Medical School,University of South China,Changsha 410004,Hunan Province,China [6]Laboratory of Electron Microscopy,Ultrastructural Pathology Center,Peking University First Hospital,Beijing 100034,China [7]Diabetes Research Center,Chinese Academy of Medical Sciences,Beijing 100005,China

出　　处：《World Journal of Gastroenterology》2025年第15期79-95,共17页世界胃肠病学杂志(英文)

基　　金：Supported by the Beijing Municipal Natural Science Foundation,No.7212068;the National Natural Science Foundation of China,No.81900747.

摘　　要：BACKGROUND The integrity and functionality of the hepatic microcirculation are essential for maintaining liver health,which is influenced by sex and genetic background.Understanding these variations is crucial for addressing disparities in liver disease outcomes.AIM To investigate the sexual dimorphism and genetic heterogeneity of liver microcirculatory function in mice.METHODS We assessed hepatic microhemodynamics in BALB/c,C57BL/6J,and KM mouse strains using laser Doppler flowmetry and wavelet analysis.We analyzed the serum levels of alanine transaminase,glutamic acid aminotransferase,total bile acid,total protein,alkaline phosphatase,and glucose.Histological and immunohistochemical staining were employed to quantify microvascular density and the expression levels of cluster of differentiation(CD)31,and estrogen receptorα,andβ.Statistical analyses,including the Mantel test and Pearson correlation,were conducted to determine the relationships among hepatic function,microcirculation,and marcocirculation between different sexes and across genetic backgrounds.RESULTS We identified sex-based disparities in hepatic microhemodynamics across all strains,with males exhibiting higher microvascular perfusion and erythrocyte concentration,but lower blood velocity.Strain-specific differences were evident,particularly in the endothelial oscillatory characteristics of the erythrocyte concentration.No sexdependent differences in estrogen receptor expression were observed,while significant variations in CD31 expression and microvascular density were observed.The correlations highlighted relationships between hepatic microhemodynamics and liver function indicators.CONCLUSION Our findings indicate the influence of genetic and sex differences on hepatic microcirculation and liver function,highlighting the necessity of incorporating both genetic background and sex into hepatic physiology studies and potential liver disease management strategies.

关 键 词：Hepatic microhemodynamics Sex differences Mouse strains Biological oscillators Hepatic microcirculation 

分 类 号：R575[医药卫生—消化系统]