Correlation between the interleukin-36 subfamily and gut microbiota in patients with liver cirrhosis:Implications for gut-liver axis imbalance  

作　　者：Yi-Zhi Pan Wan-Ting Chen Hao-Ran Jin Zhen Liu Ying-Ying Gu Xin-Ruo Wang Jue Wang Jing-Jing Lin Yan Zhou Lan-Man Xu 

机构地区：[1]Department of Infectious Diseases and Liver Diseases,Lihuili Hospital of Ningbo University,Ningbo 315000,Zhejiang Province,China [2]Department of Infectious Diseases and Liver Diseases,People’s Hospital of Ningbo University,Ningbo 315000,Zhejiang Province,China [3]Department of Rheumatology and Immunology,Ningbo Hangzhou Bar Hospital,Ningbo 315000,Zhejiang Province,China

出　　处：《World Journal of Hepatology》2025年第4期75-84,共10页世界肝病学杂志(英文)

基　　金：Supported by Key Project of the Ningbo Natural Science Foundation,Zhejiang Province,China,No.2022J253;Key Technology R&D Project of Ningbo City,No.2023Z208;Traditional Chinese Medicine project,Zhejiang Province,No.2024ZF028;the Key Project of Health Science and Technology Foundation,Zhejiang Province,China,No.WKJ-ZJ-2551.

摘　　要：BACKGROUND Liver cirrhosis(LC)affect millions of people worldwide.The pathogenesis of cirrhosis involves complex interactions between immune responses and gut microbiota.Recent studies have highlighted the role of the interleukin-36(IL-36)subfamily in inflammation and immune regulation.However,the relationship between serum IL-36 subfamily levels and gut microbiota in cirrhosis patients remains unclear.This study aimed to explore the clinical significance of serum IL-36 subfamily levels and their association with gut microbiota in cirrhosis patients.AIM To explore the clinical significance of serum IL-36 subfamily levels and their relationship with gut microbiota among cirrhosis patients.METHODS Sixty-one cirrhosis patients were enrolled from Lihuili Hospital of Ningbo University from May 2022 to November 2023 as the LC group and 29 healthy volunteers as the healthy control(HC)group.The serum expressions of IL-36α,IL-36β, IL-36γ, IL-36Ra, and IL-38 were measured through ELISA, while 16S rRNA gene sequencing was employed torate microbial community in human fecal samples.RESULTSThe serum levels of IL-36α, IL-36γ, IL-36Ra, and IL-38 in the LC group remarkably exceeded those in the HC group(P < 0.05). IL-36α, IL-36γ, and IL-38 were related positively to the Child-Pugh score (P < 0.05) and prominentlyexceeded those in the Child-Pugh C group (P < 0.05). The absolute abundance of harmful bacteria (Bacteroides,Bifidobacterium, Faecalibacterium) remarkably rose, while the beneficial bacteria (Firmicutes, Bacteroides, Escherichia-Shigella) notably decreased in the LC group (P < 0.05). IL-36α, IL-36γ, and IL-38 related positively to Lactobacillus(P < 0.05), while IL-38 negatively related to Fusicatenibacter (P < 0.05).CONCLUSIONIL-36γ and IL-38 show promise as potential biomarkers for LC progression, but further validation is required.

关 键 词：Interleukin-36 Interleukin-36γ Interleukin-38 Liver cirrhosis Gut microbiota 

分 类 号：R575.2[医药卫生—消化系统]