富硒绿茶提取物对肠道菌群的调节作用  

Regulation Effects of Selenium⁃Enriched Green Tea Extracts on Intestinal Flora

作　　者：陶会 徐婵 宋杰玉 李进 滕春丽 潘灿平[3] 杨鸿波 魏绍峰[1] TAO Hui;XU Chan;SONG Jieyu;LI Jin;TENG Chunli;PAN Canping;YANG Hongbo;WEI Shaofeng(School of Public Hygiene and Health,Guizhou Medical University,Guiyang 550025,Guizhou,China;Guizhou Jianande Technology Co.,Ltd.,Guiyang 550014,Guizhou,China;College of Science,China Agricultural University,Beijing 100094,China)

机构地区：[1]贵州医科大学公共卫生与健康学院,贵州贵阳550025 [2]贵州健安德科技有限公司,贵州贵阳550014 [3]中国农业大学理学院,北京100094

出　　处：《食品研究与开发》2025年第7期9-16,共8页Food Research and Development

基　　金：国家自然科学基金项目(21966011);中央引导地方科技发展资金项目(2022ZY0049);贵州省科技计划项目(黔科中引地[2021]4039)。

摘　　要：探究富硒绿茶提取物对肠道菌群失调小鼠的影响,采用头孢曲松钠进行肠道菌群失调模型的建立,检测小鼠肠组织中分泌型免疫球蛋白A(secretory immunoglobulin A,SIgA)、脂多糖(lipopolysaccharide,LPS)和小鼠粪便中短链脂肪酸(short-chain fatty acids,SCFAs)含量,并通过16S rRNA高通量测序检测富硒绿茶提取物对小鼠肠道菌群的调节作用。结果表明,富硒绿茶提取物显著提高肠道菌群失调小鼠回肠SIgA含量、小鼠粪便中SCFAs含量,降低小鼠结肠组织中LPS含量,相比普通绿茶,富硒绿茶具有更强的肠道免疫调节和抗炎优势。高通量测序结果显示,富硒绿茶提取物可以显著调节头孢曲松钠引起的小鼠肠道微生物多样性改变,抑制变形菌和放线菌的生长和促进毛螺旋菌、阿克曼菌、拟杆菌等有益菌的繁殖,因此富硒绿茶提取物具有调节小鼠肠道菌群的作用。To explore the effects of selenium-enriched green tea extract on mice with intestinal flora dysregulation,ceftriaxone sodium was used to establish the intestinal flora dysregulation model.Changes in the levels of secretory immunoglobulin A(SIgA)and lipopolysaccharide(LPS)in the intestinal tissues,as well as shortchain fatty acids(SCFAs)in mouse feces,were detected.The regulatory effect of selenium-enriched green tea extract on intestinal flora in mice was detected using 16S rRNA high-throughput sequencing.The results showed that selenium-enriched green tea extract significantly increased the SIgA content in the ileum of intestinal flora-dysregulated mice,enhanced SCFAs content in feces,and decreased the LPS content in colon tissue.Compared to ordinary green tea,selenium-enriched green tea extract exhibited stronger intestinal immunomodulatory and anti-inflammatory effects.High-throughput sequencing results showed that selenium-enriched green tea extract could significantly regulate the changes in intestinal microbial diversity induced by ceftriaxone sodium in mice.It inhibited the growth of Proteobacteria and Actinomyces while promoting the proliferation of beneficial bacteria such as Lachnospiraceae,Akkermansia,and Bacteroides.Therefore,selenium-enriched green tea extract could effectively regulate the intestinal microflora of mice.

关 键 词：富硒绿茶 肠道菌群 16S rRNA高通量测序 硒 头孢曲松钠 

分 类 号：TS201.4[轻工技术与工程—食品科学]