一株精氨酸支原体的分离培养及分子生物学鉴定  

Isolation,Cultivation and Molecular Identification of a Strain of Mycoplasma arginine

作　　者：杨康 马春江 李海利 杨夷平 段进刚 古丽巴哈尔·卡德尔 陈建豪 张国新 杨帆 YANG Kang;MA Chunjiang;LI Haili;YANG Yiping;DUAN Jingang;GURI BAHAR·Cardle;CHEN Jianhao;ZHANG Guoxin;YANG Fan(Hami Animal Disease Prevention and Control Center,Hami,Xinjiang 839000,China)

机构地区：[1]哈密市动物疫病预防控制中心,新疆哈密839000

出　　处：《草食家畜》2025年第2期20-27,共8页Grass-Feeding Livestock

基　　金：新疆维吾尔自治区“三农”骨干人才培养项目“新疆哈密市牛羊常见病标准化防控技术的推广与应用”(2022SNGGNT032)。

摘　　要：【目的】旨在探究新疆哈密市伊州区某养殖户羊发病死亡的原因。【方法】采集病死羊的肺组织,进行分离培养、实时荧光定量PCR鉴定、普通PCR扩增以及基因测序分析。【结果】在改良的PPLO固体培养基上培养出了支原体;实时荧光定量PCR检测结果为支原体感染阳性;普通PCR扩增及基因测序,分离菌株与GenBank数据库中的精氨酸支原体同源性高达100%。【结论】研究结果表明,该养殖户羊发病死亡是由精氨酸支原体感染导致。【Objective】In order to investigate the causes of illness and death of sheep in a household in Yizhou district,Hami City.【Methods】Lung tissue samples of dead sheep were collected,and bacterial isolation and cultivation,real-time fluorescent quantitative PCR,conventional PCR as well as gene sequencing and analysis were performed.【Results】Mycoplasma was cultured successfully on the surface of improved PPLO solid medium.Positive results of Mycoplasma infection were found by real-time fluorescent quantitative PCR.According to PCR amplification and gene sequencing results,the sequence of the isolate had 100% homology with that of Mycoplasma arginine registered in GenBank database.【Conclusion】A Hami strain of Mycoplasma arginine was successfully isolated.

关 键 词：精氨酸支原体 分离培养 PCR 16SrRNA 基因测序 

分 类 号：S852.62[农业科学—基础兽医学]