中药单体芹菜素联合顺铂对人结直肠癌细胞HCT116和SW480增殖、凋亡的影响及机制研究  

Effect and mechanism of the traditional Chinese medicine monomer apigenin combined with cisplatin on the proliferation and apoptosis of human colorectal cancer cells

作　　者：董雷 杨峰 郭家兴 王雪 王艺颖 黄梅 张威 罗林山 张晶芳 李永伟[3] 巩跃生 DONG Lei;YANG Feng;GUO Jiaxing;WANG Xue;WANG Yiying;HUANG Mei;ZHANG Wei;LUO Linshan;ZHANG Jingfang;LI Yongwei;GONG Yuesheng(Central Laboratory,Zhengzhou Anorectal Hospital,Zhengzhou 450004;Second Clinical Medical College,Henan University of Chinese Medicine;Inspection Center,Henan Province Hospital of Traditional Chinese Medicine,China)

机构地区：[1]郑州市大肠肛门病医院中心实验室,河南郑州450004 [2]河南中医药大学第二临床医学院 [3]河南省中医院检验中心

出　　处：《胃肠病学和肝病学杂志》2025年第3期383-389,共7页Chinese Journal of Gastroenterology and Hepatology

基　　金：河南省中医药科学研究专项课题项目(2023ZY1027,2024ZY3107);国家中医药管理局人才交流中心中医药高质量发展协同创新转化工程自主立项项目(CXZH202303)。

摘　　要：目的探究中药单体芹菜素联合顺铂对人结直肠癌细胞HCT116和SW480增殖、凋亡的影响及相关机制。方法采用结直肠癌细胞系HCT116和SW480为实验细胞,在确定芹菜素和顺铂处理细胞的合适剂量后,设置空白对照组、芹菜素组、顺铂组、芹菜素+顺铂组4个实验组,利用CCK-8实验、克隆形成实验和流式细胞术实验分别检测了结直肠癌细胞增殖、凋亡的影响;采用Western blotting技术检测增殖、凋亡及Wnt/β-catenin信号通路相关蛋白的表达水平。结果40μmol/L芹菜素、20μmol/L顺铂可抑制细胞的活力,促进细胞凋亡。相对其他组,芹菜素+顺铂组表现出更好的抑制细胞活力效果,更能够促进细胞凋亡,并可以调控Wnt/β-catenin信号通路。结论芹菜素可用于结直肠癌药物的研发,为开发相应的肿瘤治疗药物提供科学依据。Objective To investigate the effect and mechanism of the traditional Chinese medicine monomer apigenin combined with cisplatin on the proliferation and apoptosis of human colorectal cancer cells.Methods Two typical colorectal cancer cell lines,HCT116 and SW480,were utilized as experimental subjects.Following the determination of optimal dosages for apigenin and cisplatin,four experimental groups were established:a blank control group,an apigenin group,a cisplatin group and apigenin+cisplatin group receiving both apigenin and cisplatin.The effects on cell proliferation and apoptosis were assessed using CCK-8 assays,colony formation assays and flow cytometry.Western blotting was employed to evaluate the expression levels of proteins associated with proliferation,apoptosis and the Wnt/β-catenin signaling pathway.Results Treatment with 40μmol/L of apigenin and 20μmol/L of cisplatin significantly inhibited cell viability and promoted apoptosis.Notably,the combination of apigenin and cisplatin demonstrated superior inhibitory effects on cell viability and enhanced apoptosis compared to the other groups,while also modulating the Wnt/β-catenin signaling pathway.Conclusion Apigenin shows potential for the development of novel therapeutic agents for colorectal cancer,providing a scientific foundation for the creation of effective tumor treatment drugs.

关 键 词：芹菜素 顺铂 结直肠癌细胞 增殖 凋亡 

分 类 号：R735.3[医药卫生—肿瘤]