Zfp260对牙周炎破骨细胞分化影响的实验研究  

Experimental study of the effect of Zfp260 on the differentiation of osteoclasts in periodontitis

作　　者：贡若男 王佐林[1] GONG Ruonan;WANG Zuolin(Shanghai Engineering Research Center of Tooth Restoration and Regeneration&Tongji Research Institute of Stomatology&Department of Oral Implantology,Shanghai Tongji Stomatological Hospital and Dental School,Tongji University,Shanghai 200072,China)

机构地区：[1]上海市同济口腔医院口腔种植科,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学口腔医学研究所,上海200072

出　　处：《口腔颌面外科杂志》2025年第2期91-97,共7页Journal of Oral and Maxillofacial Surgery

基　　金：科技部重点研发专项(2018YFE0202200);国家自然科学基金(81670962)。

摘　　要：目的:探究锌指蛋白260(zinc finger protein 260,Zfp260)对牙周炎破骨细胞分化的影响。方法:将Zfp260小干扰RNA(small interfering RNA,siRNA)转染进单核/巨噬细胞系RAW264.7,转染后的细胞在核因子κB受体活化因子配体(receptor activator of nuclear factorκB ligand,RANKL)诱导7 d后,通过抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase,TRAP)染色观察破骨细胞的形成,实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)检测破骨细胞分化标志基因的表达水平。基于Zfp260 flox/flox小鼠(对照组)及基因型为Zfp260 flox/flox Lyz2-cre-的髓系细胞条件性敲除Zfp260基因(Zfp260 conditional knockout,Zfp260 CKO)小鼠(实验组)构建牙周炎模型,通过Micro-CT和组织学染色评价牙槽骨骨质破坏情况。结果:Zfp260在RAW264.7细胞向破骨细胞分化过程中表达上调;沉默Zfp260表达后,RAW264.7细胞破骨分化能力下降,破骨分化标志基因表达下调(P<0.05);Zfp260 CKO组小鼠牙周炎侧骨吸收程度较轻。结论:Zfp260正向调控破骨细胞形成促进骨吸收,提示Zfp260可能成为牙周炎骨吸收的防治靶点。Objective:To research the role of zinc finger protein 260(Zfp260)in osteoclast differentiation under the periodontitis microenvironment.Methods:Small interfering RNA(siRNA)was transfected into the monocyte/macrophage cell line RAW264.7.The transfected cells were induced into osteoclasts by receptor activator of nuclear factorκB ligand(RANKL)for 7 days.Differentiated cells were harvested for tartrate resistant acid phosphatase(TRAP)staining.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the mRNA expression level of osteoclast differentiation-related genes.In vivo,experimental periodontitis models were established in Zfp260 flox/flox mice(Control group)and Zfp260 flox/flox Lyz2-cremyeloid cell conditional Zfp260-knockout mice(Zfp260 cko,Experimental group).The Micro-CT scan and histological staining were used to analyze alveolar bone resorption.Results:Zfp260 was up-regulated during osteoclast genesis.The knockdown of Zfp260 inhibited the differentiation of RAW264.7 cells into osteoclasts,and the expression of osteoclast-related factors was down-regulated(P<0.05).In vivo,the bone resorption was significantly decreased on the experimental side in Zfp260CKO mice.Conclusion:Zfp260 regulates the osteoclast differentiation ability,which will provide a new target for solving periodontitis.

关 键 词：牙周炎 牙槽骨吸收 破骨细胞 锌指蛋白260 

分 类 号：R782[医药卫生—口腔医学]