EGR1通过激活lnc-HCG11/Wnt/β-catenin通路促进牙周膜细胞的成骨分化  

EGR1 promotes osteogenic differentiation of periodontal ligament cells by activating the lnc-HCG11/Wnt/β-catenin pathway

作　　者：李成 隆湘凤 周琴 徐方方 黄学成 毛加团 LI Cheng;LONG Xiangfeng;ZHOU Qin;XU Fangfang;HUANG Xuecheng;MAO Jiatuan(Department of Stomatology,Jing'an District Institute of Dantal Diseases,Shanghai 200040;Department of Stomatology,Guangnan People's Hospital,Wenshan 663300,China)

机构地区：[1]上海市静安区牙病防治所口腔科,上海200040 [2]广南县人民医院口腔科,文山州663300

出　　处：《口腔颌面外科杂志》2025年第2期116-122,共7页Journal of Oral and Maxillofacial Surgery

基　　金：2022年静安区医学科研课题(2022MS18)。

摘　　要：目的:探讨早期生长应答因子1(early growth response gene 1,EGR1)是否通过调控长链非编码RNA人白细胞抗原复合物11(long non-coding RNA human leukocyte antigen complex group 11,lnc-HCG11)和无翅基因/β-连环蛋白(wingless/β-catenin,Wnt/β-catenin)通路影响人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)的成骨分化。方法:通过载体转染过表达和干扰EGR1和lnc-HCG11在hPDLSCs中的表达,通过β-catenin抑制剂IWR-1抑制该通路的活化水平,实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)与蛋白质印迹法检测干预效果。对上述处理的hPDLSCs进行成骨诱导后,利用RT-qPCR检测成骨基因Runt相关转录因子2(runt-related transcription factor 2,RUNX2)、Ⅰ型胶原α1(collagen typeⅠα1,COL1A1)、骨桥蛋白(osteopontin,OPN)、骨钙素(osteocalcin,OCN)的表达水平,茜素红染色检测细胞的矿化水平。结果:过表达EGR1促进了hPDLSCs中lnc-HCG11的表达,过表达lnc-HCG11促进hPDLSCs中RUNX2、COL1A1、OPN、OCN的表达水平及矿化水平,沉默lnc-HCG11抑制EGR1的促成骨分化作用,加入IWR-1后抑制了活性β-catenin的表达,同时削弱了lnc-HCG11对hPDLSCs的促成骨分化作用。结论:EGR1通过激活lnc-HCG11/Wnt/β-catenin通路促进hPDLSCs的成骨分化。Objective:To investigate the effect of early growth response gene 1(EGR1)on osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs)by regulating long non-coding RNA human leukocyte antigen complex group 11(lnc-HCG11)and wingless/β-catenin(Wnt/β-catenin)pathway.Methods:The expression levels of EGR1 and lnc-HCG11 were changed in hPDLSCs through gene overexpression and interference assays,while the activation level of the Wnt/β-catenin pathway was altered by theβ-catenin inhibitor IWR-1.Subsequently,real-time quantitative polymerase chain reaction(RTqPCR)and Western blotting experiments were conducted to validate the intervention effect.After osteogenic induction,RTqPCR was used to detect the expression levels of osteogenic genes runt-related transcription factor 2(RUNX2),collagen typeⅠα1(COL1A1),osteopontin(OPN),osteocalcin(OCN),and alizarin red staining was used to detect the mineralization level of cells.Results:The overexpression of EGR1 was observed to promote the expression of lnc-HCG11 in hPDLSCs,while the overexpression of lnc-HCG11 promoted the expression levels of RUNX2,COL1A1,OPN,OCN,and mineralization in hPDLSCs.The pro-osteogenic effect of EGR1 was inhibited by silencing lnc-HCG11,while treatment with IWR-1 suppressed the expression of activeβ-catenin and weakened the pro-osteogenic effect of lnc-HCG11 on hPDLSCs.Conclusion:EGR1 promotes osteogenic differentiation of hPDLSCs by activating the lnc-HCG11/Wnt/β-catenin pathway.

关 键 词：牙周炎 人牙周膜干细胞 早期生长应答因子1 lncRNA HCG11 成骨分化 

分 类 号：R782[医药卫生—口腔医学]