SOX10在三阴性乳腺癌中的表达及其与肿瘤免疫浸润特征的相关性  

Association between the expression of SOX10 in triple negative breast cancer and its tumor infiltrated immune cell

作　　者：陈宝强 李东海 郝婷 闫秀英 徐海成 王芙蓉[4] CHEN Baoqiang;LI Donghai;HAO Ting;YAN Xiuying;XU Haicheng;WANG Furong(Department of Pathology,The First People's Hospital of Lanzhou City,Lanzhou 730050,China;Department of Personnel,The First People's Hospital of Lanzhou City,Lanzhou 730050,China;Department of Medical Insurance,The Second Hospital of Lanzhou University,Lanzhou 730030,China;Department of Pathology,The Second Hospital of Lanzhou University,Lanzhou 730030,China)

机构地区：[1]兰州市第一人民医院病理科,甘肃兰州730050 [2]兰州市第一人民医院人事科,甘肃兰州730050 [3]兰州大学第二医院医保科,甘肃兰州730030 [4]兰州大学第二医院病理科,甘肃兰州730030

出　　处：《兰州大学学报(医学版)》2025年第3期8-13,共6页Journal of Lanzhou University(Medical Sciences)

基　　金：甘肃省自然科学基金资助项目(22JR5RA1063)。

摘　　要：目的探讨SOX10的表达与三阴性乳腺癌(TNBC)的临床病理特征以及肿瘤间质中CD8^(+)T细胞浸润之间的关系,阐明SOX10在TNBC中的表达及作用。方法收集2018年1月1日—2024年5月30日兰州市第一人民医院术后诊断为非特殊型浸润性乳腺癌手术标本85例,其中TNBC 45例。通过免疫组织化学染色技术,检测癌组织中SOX10、细胞角蛋白、表皮生长因子受体的表达情况,以及肿瘤间质中CD8的表达水平;利用TIMER数据库,分析SOX10基因与基底样乳腺癌的纯度以及肿瘤间质中CD8^(+)T细胞浸润之间的关系。结果SOX10仅在TNBC组织中表达,表达率为60%(27/45),而在所有非三阴性浸润性导管癌组织中不表达(P<0.001),在TNBC中的SOX10表达与患者的年龄呈相关性(P<0.05),但与病理分级、TNM分期、肿瘤的最大直径以及淋巴结转移情况无关(P>0.05)。SOX10阳性的TNBC中,基底样乳腺癌占比81.48%(22/27)。与SOX10阴性的TNBC比较,SOX10阳性的TNBC肿瘤间质中CD8^(+)T细胞的浸润数量明显减少。结论SOX10是鉴别诊断TNBC的敏感标志物,倾向于年轻患者的癌组织中表达;SOX10阳性的TNBC肿瘤组织很可能主要源自乳腺的基底细胞,SOX10可抑制CD8^(+)T细胞在TNBC肿瘤间质中的浸润。Objective To elucidate the expression and significance of SOX10 in triple-negative breast cancer(TNBC),this study investigated the relationship between SOX10 expression and the clinicopathological fea-tures of TNBC as well as the infiltration of CD8^(+)T cells in the tumor stroma.Method We collected 85 breast cancer surgical specimens diagnosed as non-specific invasive ductal carcinoma in The First People's Hospital of Lanzhou City from January 1,2018 to May 30,2024,postoperatively,including 45 cases of TNBC.Using the immunohistochemical staining techniques,we examined the expression of SOX10,cytokeratin5/6,epidermal growth factor receptor in the cancerous tissues,and the expression level of CD8 in the tumor stro-ma.Furthermore,by utilizing the TIMER database,we analyzed the relationship between SOX10 gene expres-sion and the purity of basal-like breast cancer,as well as the infiltration of CD8^(+)T cells in the tumor stroma.Result SOX10 was exclusively expressed in triple-negative breast cancer(TNBC)tissues,with no expression observed in all non-triple-negative invasive ductal carcinoma tissues.The expression rate of SOX10 in TNBC was 60%(27/45).The expression of SOX10 in TNBC was correlated with the ages of patients(P<0.05),but not related to the pathological grade,TNM stage,the maximum diameter of the tumor,or the status of lymph node metastasis.In SOX10-expressing TNBC,the proportion of basal-like breast cancer was as high as 81.48%(22/27).Compared with TNBC that did not express SOX10,the number of CD8^(+)T cell infiltration in the tumor stroma of SOX10-expressing TNBC was significantly reduced.Conclusion SOX10 is a sensitive marker for differential diagnosis of TNBC,and tends to express in the cancer tissues of younger patients.In addition,the tumor tissues of SOX10-expressing TNBC are likely to mainly originate from the basal cells of the breast,and the expression of SOX10 may inhibit the infiltration of CD8^(+)T cells in the TNBC tumor stroma.

关 键 词：SOX10 三阴性乳腺癌 临床病理特征 基底样乳腺癌 CD8~+T细胞浸润 

分 类 号：R737.9[医药卫生—肿瘤]