胃癌m^(7)G相关微小RNA预后模型的构建与验证  

Construction and validation of a prognostic model of m^(7)G-associated microRNA in gastric cancer

作　　者：余春燕 刘涛 李亚西 杨晓晓 黄翌楚 李桐桐 闵光涛[2] 姜雷[2] YU Chunyan;LIU Tao;LI Yaxi;YANG Xiaoxiao;HUANG Yichu;LI Tongtong;MIN Guangtao;JIANG Lei(The First Clinical Medical School,Lanzhou University,Lanzhou 730000,China;Department of General Surgery,The First Hospital of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一临床医学院,甘肃兰州730000 [2]兰州大学第一医院普外科,甘肃兰州730000

出　　处：《兰州大学学报(医学版)》2025年第3期47-58,80,共13页Journal of Lanzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目(82060527);兰州市科技发展指导性计划资助项目(2020-ZD-66)。

摘　　要：目的 探讨N7-甲基鸟苷(m^(7)G)相关微小核糖核酸(miRNA)在胃癌预后模型中的预测价值。方法 构建胃癌m^(7)G相关miRNA预后模型,根据模型计算风险评分临界值,将胃癌患者分为高风险组和低风险组,比较2组患者的总生存时间。进一步分析高、低风险组在肿瘤免疫逃逸和对免疫治疗药物的敏感性差异,以及肿瘤突变负荷与免疫治疗反应之间的相关性。结果 通过构建模型,确定了由10个m^(7)G相关miRNA构成的预后模型;低风险组患者的总生存期比高风险组长;回归分析显示,风险评分可作为独立预后因素(风险评分为1.009,风险比为1.003~1.015,P<0.05)。基于风险评分和临床病理因素构建列线图,预测胃癌患者1、3和5年生存率的受试者操作特征曲线下面积分别为0.753、0.768和0.749。2组间的肿瘤突变负荷及免疫治疗效果差异具有统计学意义。实时定量聚合酶链式反应结果显示,hasmir-200c、has-mir-942和has-mir-3138在肿瘤组织中低表达。结论 m^(7)G相关miRNA预后风险模型为胃癌患者生存预测及免疫治疗分层的潜在工具。Objective To investigate the predictive value of N-7 methylguanosine(m^(7)G)-related microRNA(miRNA)in gastric cancer prognosis models.Methods A m^(7)G-related miRNA prognostic model for gastric cancer was established,and the risk score computed based on this model.Patients with gastric cancer were classified into high-risk and low-risk groups,and their overall survival times were compared.Differences in tumor immune escape and sensitivity to immunotherapy drugs between high-risk and low-risk groups were further analyzed,and the correlation between tumor mutation burden and immunotherapy response was inves-tigated.Results A prognostic model consisting of 10 miRNA associated with m^(7)G was constructed.The overall survival of patients in the low-risk group was significantly longer compared to that of the high-risk group.Regression analysis indicated that the risk score could serve as an independent prognostic factor(risk score=1.009,hazard ratio=1.003-1.015,P<0.05).Based on the risk score and clinicopathological factors,the area under the subject operation characteristic curve for predicting 1-year,3-year,and 5-year survival rates of gastric cancer patients was 0.753,0.768,and 0.749,respectively.Significant differences were observed in tu-mor mutation burden and the effectiveness of immunotherapy between the two groups.Real-time quantitative PCR results revealed that hsa-mir-200c,hsa-mir-942,and hsa-mir-3138 were significantly downregulated in tumor tissues.Conclusion The m^(7)G-related miRNA prognostic risk model serves as a potential tool for sur-vival prediction and immunotherapy stratification in gastric cancer patients.

关 键 词：N7-甲基鸟苷 甲基化 微小核糖核酸 预后 肿瘤突变负荷 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]