A reliable,simple and cost-efficient UPLC-vwd method for simultaneously determining tocopherol and tocotrienol in peanut seeds  

作　　者：Zekai Tian Yue Zhang Chenshuo Sun Liying Yan Yanping Kang Dongxin Huai Yuning Chen Xin Wang Qianqian Wang Huifang Jiang Boshou Liao Zhihui Wang Yong Lei 

机构地区：[1]The Key Laboratory of Biology and Genetic Improvement of Oil Crops,the Ministry of Agriculture and Rural Affairs,Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Wuhan,430062,China [2]National Key Laboratory of Crop Genetic Improvement,National Center of Crop Molecular Breeding Technology,National Center of Oil Crop Improvement(Wuhan),Huazhong Agricultural University,Wuhan,430070,China

出　　处：《Oil Crop Science》2025年第1期70-78,共9页中国油料作物学报(英文版)

基　　金：supported by the project of the development for highquality seed industry of Hubei province(HBZY2023B003);the Key Area Research and Development Program of Hubei Province(2021BBA077);the Agricultural Science and Technology Innovation Program.

摘　　要：Vitamin E is an essential micronutrient that is abundant in peanut seeds.However,the absence of a rapid and reliable method for determining its content has impeded advancements in peanut quality improvement.In this study,we developed an efficient ultra-performance liquid chromatography variable wavelength detector(UPLCvwd)method for quantifying vitamin E content in peanut seeds,capable of detecting four tocopherols and four tocotrienols.Compared to traditional methods,this approach is simpler,more efficient,and highly accurate.By comparing two sample preparation techniques,it was found that slicing better reflects the true vitamin E content than grinding,as it minimizes losses caused by mechanical pressure.The method demonstrated robust stability and accuracy in both repeatability tests and spiked recovery tests,showing no significant differences compared to the national standard method.The study revealed that the cotyledons of peanuts are the primary storage site for vitamin E,being rich inγ-andα-tocopherols,which together account for over 90%of the total vitamin E content.In contrast,the vitamin E content in the seed coat is considerably lower.Therefore,the optimized detection method minimizes seed coat interference during sample preparation,ensuring the accuracy of the results.In summary,the UPLC-vwd method developed in this study is a promising tool for determining vitamin E content and supports the improvement of peanut quality.

关 键 词：PEANUT Seed Vitamin E UPLC-vwd 

分 类 号：S565.2[农业科学—作物学]