癫痫患儿血清LncRNA-NEAT1 miR-93-5p表达水平及其与脑电图严重程度和预后的关系  

Relationship between expression of serum LncRNA-NEAT1 and miR-93-5p in children with epilepsy and EEG abnormalities and prognosis

作　　者：刘丹丹 冯娇娇 邢雅杰 LIU Dandan;FENG Jiaojiao;XING Yajie(Pediatrics Department of Handan Central Hospital Hebei,Handan 056001,China;Pediatrics Department of Handan First Hospital Hebei,Handan 056001,China)

机构地区：[1]邯郸市中心医院儿科,河北邯郸056001 [2]邯郸市第一医院儿科,河北邯郸056001

出　　处：《安徽医学》2025年第4期439-444,共6页Anhui Medical Journal

摘　　要：目的探讨长链非编码RNA核富集转录体1(LncRNA-NEAT1)和微小RNA-93-5p(miR-93-5p)在癫痫儿童血清中的表达及其与脑电图异常和预后的关系。方法前瞻性选取2019年5月至2021年5月在邯郸市中心医院儿科治疗的癫痫儿童99例为观察组,根据异常脑电图严重程度分为脑电图轻度异常组37例、中度异常组22例、重度异常组40例;所有患者随访2年,根据随访结果分为预后不良组38例、预后良好组61例。另取同期在本院进行健康体检且一般资料与癫痫儿童相匹配的健康儿童99例作为对照组。采用定量逆转录聚合酶链反应(qRT-PCR)法检测所有受试者血清LncRNA-NEAT1、miR-93-5p表达水平;采用Spearman法分析LncRNA-NEAT1、miR-93-5p与脑电图异常严重程度的关系;采用logistic回归分析癫痫儿童脑电图重度异常及预后不良的影响因素。结果观察组血清LncRNA-NEAT1高于对照组,miR-93-5p低于对照组(P<0.05);不同脑电图异常程度的癫痫儿童血清LncRNA-NEAT1、miR-93-5p水平两两比较,差异均有统计学意义(P<0.05);LncRNA-NEAT1与脑电图异常严重程度呈正相关(rs=0.801,P<0.05),miR-93-5p与脑电图异常严重程度呈负相关(rs=-0.661,P<0.05);病程长、发作频率>1次/天、LncRNA-NEAT1高表达均是癫痫儿童脑电图重度异常的危险因素(P<0.05),miR-93-5p高表达是其保护因素(P<0.05)。预后不良组血清LncRNANEAT1水平高于预后良好组,miR-93-5p水平低于预后良好组(P<0.05);LncRNA-NEAT1高表达是癫痫儿童预后不良的危险因素,miR-93-5p高表达是其保护因素(P<0.05)。结论癫痫儿童血清LncRNA-NEAT1水平升高,与脑电图异常严重程度呈正相关,是预后不良的危险因素;这类患儿miR-93-5p水平降低,与脑电图异常严重程度呈负相关,是预后不良的保护因素。Objective To investigate the relationship between the expression of long non-coding RNA nuclear enriched abundant transcript 1(LncRNA-NEAT1)and microRNA-93-5p(miR-93-5p)in the serum of epileptic children and EEG abnormalities and prognosis.Methods A total of 99 children with epilepsy who were treated in the Department of Pediatrics,Handan Central Hospital from May 2019 to May 2021 were regarded as the observation group.According to the severity of abnormal EEG,there were 37 cases recruited in the mild abnormal EEG group,22 cases in the moderate abnormal EEG group,and 40 cases in the severe abnormal EEG group.Patients were followed up for two years,and were divided into poor prognosis group(38 cases)and good prognosis group(61 cases)according to the follow-up results.Another 99 healthy children who underwent health examinations in our hospital and matched general information with epilepsy children were collected as the control group.qRT-PCR was applied to detect the expression levels of serum LncRNA NEAT1 and miR-93-5p in all subjects.Spearman method was used to analyze the correlation between LncRNA-NEAT1 and miR-93-5p and the severity of EEG abnormalities.Logistic regression was applied to analyze the influencing factors of severe EEG abnormalities and poor prognosis in children with epilepsy.Re⁃sults The serum LncRNA NEAT1 level in the observation group was obviously higher than that in the control group,and miR-93-5p level was obviously lower than that in the control group(P<0.05);the serum levels of LncRNA-NEAT1 and miR-93-5p in epileptic children with different degrees of EEG abnormalities were statistically different(P<0.05);LncRNA-NEAT1 was positively correlated with the severity of EEG abnormalities(r=0.801,P<0.05),while miR-93-5p was negatively correlated with the severity of EEG abnormalities(r=-0.661,P<0.05);Long course of disease,seizure frequency>1 time/day,and high expression of LncRNA NEAT1 were all risk factors for severe abnormalities in electroencephalogram in children with epilepsy(P<0.05),wh

关 键 词：长链非编码RNA核富集转录体1 微小RNA-93-5p 癫痫 脑电图异常 预后 

分 类 号：R742.1[医药卫生—神经病学与精神病学]