高效液相色谱法测定香波中香紫苏内酯的含量  

Determination of Sclareolide in Shampoo by HPLC Method

作　　者：郭梅燕 王云云 瞿欣 GUO Meiyan;WANG Yunyun;QU Xin(Ashland LLC,Shanghai 200233,China)

机构地区：[1]亚什兰(中国)投资有限公司,上海200233

出　　处：《香料香精化妆品》2025年第2期144-148,共5页Flavour Fragrance Cosmetics

摘　　要：建立了香紫苏内酯的高效液相色谱检测方法。利用Agilent 1260 Infinity II液相色谱仪,经二极管阵列检测器进行全波长扫描(190~400 nm),在2D光谱图上读取到香紫苏内酯的最大吸收波长为214 nm。以乙腈和水为流动相,经C18柱分离,流速1.0 mL/min,柱温35℃,检测波长214 nm,香紫苏内酯在126.4~996.0 mg/L呈良好的线性关系,相关系数(R^(2))=0.9995,满足香紫苏内酯纯度检测的需求;香紫苏内酯在58.6~199.2 mg/L与其对应的峰面积呈线性关系,且R^(2)=0.9999,可满足香紫苏内酯低含量检测需求。该法可以简便准确地对香紫苏内酯进行定量分析。In this paper,a high performance liquid chromatography method for the detection of sclareolide was established.An Agilent 1260 Infinity II liquid chromatograph with a diode array detector was used for full wavelength scanning(190-400 nm).The maximum absorption wavelength of sclareolide was 214 nm detected on the 2D spectrum.Acetonitrile and water was used as the mobile phase,the target compounds were separated by C18 column,the flow rate was 1.0 mL/min,the column temperature was 35℃,and the detection wavelength was 214 nm.The results showed that sclareolide had a good linear relationship in the range of 126.4-996.0 mg/L with the correlation coefficients(R^(2))=0.9995,which met the requirements for purity detection of sclareolide.The method showed a good linear relationship between the content and its corresponding peak area in the range of 58.6-199.2 mg/L,and R^(2) reached 0.9999,which met the requirements for low concentration detection of sclareolide.The method is simple and accurate for the quantitative analyses of sclareolide.

关 键 词：香原料 香紫苏内酯 高效液相色谱法 香波 头皮护理 

分 类 号：TS202.3[轻工技术与工程—食品科学]