微小RNA-16、B细胞淋巴瘤/白血病-2在肝细胞癌组织中的表达及与患者预后的关系  

Expression of microRNA-16 and B-cell lymphoma/leukemia-2 in hepatocellular carcinoma tissues and their relationship with prognosis of patients

作　　者：尚杰 周文艳 惠红岩[3] 苏蔚[1] 狄文玉[1] SHANG Jie;ZHOU Wenyan;HUI Hongyan;SU Wei;DI Wenyu(Department of Pathology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,He’nan,China;Department of Ultrasound Medicine,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,He’nan,China;Office of Drug Clinical Trial Institutions,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,He’nan,China)

机构地区：[1]新乡医学院第一附属医院病理科,河南新乡453100 [2]新乡医学院第一附属医院超声医学科,河南新乡453100 [3]新乡医学院第一附属医院药物临床试验机构办公室,河南新乡453100

出　　处：《癌症进展》2025年第8期903-906,共4页Oncology Progress

摘　　要：目的目的探讨微小RNA-16(miRNA-16)、B细胞淋巴瘤/白血病-2(Bcl-2)在肝细胞癌组织中的表达及与患者预后的关系。方法方法采集60例肝细胞癌手术患者的肝细胞癌组织及癌旁正常组织,采用聚合酶链反应(PCR)检测miRNA-16表达水平,采用免疫组化二步法检测Bcl-2表达情况,比较不同组织中miRNA-16的表达水平及Bcl-2表达情况。对肝细胞癌患者随访6个月,采用Logistic回归模型分析肝细胞癌患者预后的影响因素。结果肝细胞癌组织中miRNA-16表达水平明显低于癌旁正常组织,Bcl-2阳性表达率明显高于癌旁正常组织,差异有统计学意义(P﹤0.01)。多因素Logistic回归分析结果显示,临床分期为Ⅲ期、有淋巴结转移、miRNA-16低表达、Bcl-2阳性表达均是肝细胞癌患者预后不良的独立危险因素(P﹤0.05)。结论结论肝细胞癌组织中,miRNA-16表达水平较低,Bcl-2阳性表达率较高,且miRNA-16、Bcl-2表达与肝细胞癌患者的预后有关。Objective To explore the expression of microRNA-16(miRNA-16)and B-cell lymphoma/leukemia-2(Bcl-2)in hepatocellular carcinoma tissues and their relationship with prognosis of patients.Method Hepatocellular car-cinoma tissues and adjacent normal tissues from 60 patients with hepatocellular carcinoma who underwent surgery were collected.The expression level of miRNA-16 was detected by polymerase chain reaction(PCR),and the expression of Bcl-2 was detected by immunohistochemistry two-step method.The expression levels of miRNA-16 and the expression of Bcl-2 in different tissues were compared.The patients with hepatocellular carcinoma were followed up for 6 months,and the Logistic regression model was used to analyze the influencing factors of prognosis of patients with hepatocellular carcinoma.Result The expression level of miRNA-16 in hepatocellular carcinoma tissues was significantly lower than that in adjacent normal tissues,and the positive expression rate of Bcl-2 was significantly higher than that in adjacent nor-mal tissues,and the differences were statistically significant(P<0.01).Multivariate Logistic regression analysis showed that clinical stage III,lymph node metastasis,low expression of miRNA-16,and positive expression of Bcl-2 were inde-pendent risk factors for poor prognosis of patients with hepatocellular carcinoma(P<0.05).Conclusion In hepatocellu-lar carcinoma tissues,the expression level of miRNA-16 is low,and the positive expression rate of Bcl-2 is high.The ex-pression of miRNA-16 and Bcl-2 are related to the prognosis of patients with hepatocellular carcinoma.

关 键 词：肝细胞癌 微小RNA-16 B细胞淋巴瘤/白血病-2 预后 

分 类 号：R735.7[医药卫生—肿瘤]