血清中CCL14表达水平与急性脑梗死患者颈动脉斑块的相关性研究  

Study on correlation between serum CCL14 expression level and carotid atherosclerotic plaque in patients with acute cerebral infarction

作　　者：徐晓敏 邱茜茜 饶雷萍 董月 刘浩 曾萍[3] Xu Xiaomin;Qiu Qianqian;Rao Leiping;Dong Yue;Liu Hao;Zeng Ping(The Second Department of Neurology,Central Hospital of Jiaozuo Coal Group,Jiaozuo,Henan 454150,China;Department of Neurology,The First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453100,China;Department of Neurology/Hospital Infection Management,The First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453100,China)

机构地区：[1]焦作煤业(集团)有限责任公司中央医院神经内二科,河南焦作454150 [2]新乡医学院第一附属医院神经内科,河南新乡 [3]新乡医学院第一附属医院神经内科/医院感染管理科,河南新乡

出　　处：《齐齐哈尔医学院学报》2025年第8期754-759,共6页Journal of Qiqihar Medical University

基　　金：河南省医学科技攻关计划项目(LHGJ20220634)。

摘　　要：目的探讨急性脑梗死(Acute cerebral infarction,ACI)患者血清中CCL14表达水平与颈动脉斑块的相关性。方法GEO数据库下载颈动脉斑块组织及斑块旁组织中的基因表达数据集。GEO2R数据库在线分析颈动脉斑块组织中的差异表达基因。选择2022年8月-2023年1月于本医院神经内二科收治的104例ACI患者作为研究对象,作为ACI组;另选同期40名健康体检者作为对照组。ACI患者根据颈动脉超声检查结果,分为无斑块组(n=55)和斑块组(n=49),斑块组进一步分为稳定性斑块组(n=23)和不稳定性斑块组(n=26)两个亚组。所有研究对象血清中CCL14表达水平采用ELISA法检测。ACI患者血清中CCL14表达水平与颈动脉中膜厚度(Intima media thickness,IMT)和NIHSS评分的相关性采用Pearson法分析,Logistic回归分析探讨ACI患者颈动脉斑块形成的独立危险因素。ACI患者血清中CCL14表达水平诊断颈动脉斑块及不稳定性斑块的临床效能采用ROC曲线分析。结果与斑块旁组织相比,斑块组织中显著上调的基因共87个,显著下调的基因共60个。火山图,CCL14在斑块组织及斑块旁组织中的基因表达量分别为(0.78±0.12)和(0.34±0.10),与斑块旁组织相比,CCL14基因表达量在斑块组织中表达水平显著升高,差异具有统计学意义(P<0.05)。与对照组相比,CCL14表达水平在ACI组中显著升高[(34.55±3.45)pg/ml vs.(87.23±5.34)pg/ml,t=57.854,P<0.001]。无斑块组、稳定性斑块组、不稳定性斑块组血清中CCL14表达水平分别为(76.12±4.98)pg/ml、(82.88±5.17)pg/ml、(92.35±4.29)pg/ml,三组血清中CCL14表达水平比较存在显著差异(F=34.111,P<0.001),与无斑块组相比,稳定性斑块组和不稳定性斑块组血清中CCL14表达水平显著升高(P<0.05),与稳定性斑块组相比,不稳定性斑块组血清中CCL14表达水平显著升高(P<0.001)。与无斑块组相比,NIHSS评分、C反应蛋白、D-二聚体、梗死灶体积和CCL14表达水平在斑块组中�Objective To investigate the relationship between the serum expression of CCL14 and carotid artery plaque in patients with acute cerebral infarction(ACI).Methods The gene expression datasets of carotid plaque tissue and paracacular tissue was downloaded from GEO database.The GEO2R online database was used for exploring the differentially expressed genes in carotid plaque tissues.A total of 104 ACI patients(ACI group)who were admitted to second ward of neurology department in central hospital of Jiaozuo Coal Group during August 2022 and January 2023 were enrolled as study subjects,and another 40 people received healthy examination were enrolled in control group.According to the results of carotid artery ultrasonography,all ACI patients were divided into non-plaque group(n=55)and plaque group(n=49).The patients in plaque group were further divided into stable plaque group(n=23)and unstable plaque group(n=26),respectively.The serum expression level of CCL14 of all individuals was detected by ELISA method.The correlation between the serum expression level of CCL14 in ACI patients and intima media thickness(IMT)and NIHSS score were analyzed by Pearson methods.The Logistic regression analysis was conducted to explore the risk factors of carotid plaque formation in ACI patients.The clinical efficacy of serum CCL14 expression level in ACI patients in diagnosing carotid plaque formation and unstable plaque was analyzed by ROC curve.Results There were 87 significantly up-regulated genes and 60 significantly down-regulated genes in the plaque tissues compared with the paracacular tissue.The gene expression level of CCL14 in plaque and parapacal tissues were(0.78±0.12)and(0.34±0.10),respectively.Compared with paracacular tissues,the expression level of CCL14 gene in plaque tissues was significantly increased,and the difference was statistically significant(P<0.05).Compared with the control group,the serum expression level of CCL14 was significantly increased in ACI group[(34.55±3.45)pg/ml vs.(87.23±5.34)pg/ml,t=57.854,P<

关 键 词：急性脑梗死 颈动脉斑块 CCL14 ROC曲线 标志物 

分 类 号：R743.33[医药卫生—神经病学与精神病学]