MiR-508-3p靶向SULT1C4介导鼻咽癌恶性行为的机制研究  

Mechanism study of miR-508-3p targeting SULT1C4mediated malignant behavior in nasopharyngeal carcinoma

作　　者：高世俭 宗丹[2] 葛宜枝 何侠 GAO Shijian;ZONG Dan;GE Yizhi;HE Xia(First Clinical Medical College of Xuzhou Medical University,Xuzhou,Jiangsu 221004,China;Department of Radiation Therapy,Affiliated Cancer Hospital of Nanjing Medical University,Jiangsu Cancer Hospital,Jiangsu Institute of Cancer Prevention and Control,Nanjing,Jiangsu 210009,China)

机构地区：[1]徐州医科大学第一临床医学院,江苏徐州221004 [2]南京医科大学附属肿瘤医院·江苏省肿瘤医院·江苏省肿瘤防治研究所放射治疗科,江苏南京210009

出　　处：《中华肿瘤防治杂志》2025年第1期28-36,共9页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(82172804);南京市科技计划(2022SX00001663)。

摘　　要：目的探讨miR-508-3p对鼻咽癌细胞增殖、迁移、侵袭的影响,筛选出miR-508-3p的可能靶向分子SULT1C4,并探索miR-508-3p调节鼻咽癌恶性行为的分子机制。方法从基因表达综合(GEO)数据库筛选鼻咽癌组织和正常鼻咽组织检测miR-508-3p表达,应用实时荧光定量聚合酶链式反应(qRT-PCR)检测miR-508-3p在鼻咽癌细胞系5-8F和6-10B中的表达,CCK 8实验检测鼻咽癌细胞增殖情况,Transwell实验测定细胞侵袭和迁移能力。通过miRNA靶点预测网站TargetScan、miRWalk、miRDB预测出下游靶向分子SULT1C4,采用双荧光素酶报告基因验证SULT1C4与miR-508-3p之间的靶向关系,蛋白质印迹实验检测上皮-间充质转化(EMT)相关蛋白E-Cadherin、N-Cadherin和Vimentin表达水平。结果GEO数据库结果显示miR-508-3p在鼻咽癌组织呈高表达。5-8F和6-10B细胞中,敲低组miR-508-3p表达水平均低于对照组,t值分别为9.81和3.99,P值分别为0.002和0.020。CCK 8实验结果显示,5-8F细胞中,对照组和敲低组增殖能力差异有统计学意义,F组别=141.40,F时间=13248.00,F组别×时间=2251.00,均P<0.001。6-10B细胞中,对照组和敲低组增殖能力差异有统计学意义,F组别=47.16,F时间=1699.00,F组别×时间=594.70,均P<0.001。Transwell实验结果显示,5-8F细胞中,敲低组迁移率和侵袭率均低于对照组,t值分别为7.22和7.21,P值分别为0.002和0.002。6-10B细胞中呈现出同样的结果,t值分别为9.67和12.19,均P<0.001。TargetScan、miRWalk、miRDB数据库筛选出SULT1C4是miR-508-3p的靶向基因。5-8F细胞和6-10B细胞中,对照组、敲低SULT1C4组和联合敲低组SULT1C4表达水平差异有统计学意义,F值分别为16.12和127.30,P值分别为0.004和<0.001。CCK 8实验结果显示,5-8F细胞中,对照组、敲低SULT1C4组和联合敲低组增殖能力差异有统计学意义,F组别=19.67,F时间=1496.00,F组别×时间=76.49,均P<0.001;6-10B细胞中,对照组、敲低SULT1C4组和联合敲低组增殖能力差异有统�Objective To investigate the effects of miR-508-3p on the proliferation,migration and invasion of nasopharyngeal carcinoma cells,to screen the possible target molecule of miR-508-3p,SULT1C4,and to explore the molecular mechanisms by which miR-508-3p regulates the malignant behavior of nasopharyngeal carcinoma.Methods Nasopharyngeal cancer tissues and normal nasopharyngeal tissues were screened from the Gene Expression Omnibus(GEO)database to detect miR-508-3p expression.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was applied to detect miR-508-3p expression in nasopharyngeal carcinoma cell lines 5-8Fand 6-10B,and the proliferation of nasopharyngeal carcinoma cells was detected by CCK 8assay.Transwell assay was used to determine cell invasion and migration ability.The downstream targeting molecule SULT1C4 was predicted by the miRNA target prediction websites TargetScan,miRWalk,and miRDB,and the targeting relationship between SULT1C4and miR-508-3p was verified by using a dual luciferase reporter gene,and protein blotting experiments were performed to detect the epithelial mesenchymal transition(EMT)-associated proteins E-Cadherin,N-Cadherin and Vimentin expression levels.Results The GEO database results showed that miR-508-3p was highly expressed in nasopharyngeal carcinoma tissues.In 5-8Fand 6-10Bcells,the expression levels of miR-508-3p in the knockdown group were lower than those in the control group,with t-values of9.81and 3.99,and P-values of 0.002and 0.020,respectively.The CCK 8experiment results showed that there was a statistically significant difference in proliferation ability between the control group and the knockdown group in 5-8Fcells,with F_(group)=141.40,F_(time)=13 248.00,and F_(group×time)=2 251.00,all P<0.001.In 6-10Bcells,there was a statistically significant difference in proliferation ability between the control group and the knockdown group,with F_(group)=47.16,F_(time)=1 699.00,and F_(group×time)=594.70,all P<0.001.The Transwell experiment results showed that in 5-

关 键 词：鼻咽癌 miR-508-3p SULT1C4 上皮-间充质转化 增殖 迁移 侵袭 

分 类 号：R739.6[医药卫生—肿瘤]