以金铂纳米花作为双重标记的灵敏型侧向流动免疫分析  

Sensitive Lateral Flow Immunoassay Using Gold-Platinum Nanoflower as Dual Labels

作　　者：张静 吴基杰 候永成 姜采妮 陈方圆 陈赵然[1] ZHANG Jing;WU Jijie;HOU Yongcheng;JIANG Caini;CHEN Fangyuan;CHEN Zhaoran(Anhui Institute of Product Quality Supervision and Inspection,Hefei 230051,China)

机构地区：[1]安徽省产品质量监督检验研究院,安徽合肥230051

出　　处：《食品安全导刊》2025年第12期55-58,共4页China Food Safety Magazine

基　　金：国家市场监督管理总局科技创新人才计划(青年拔尖人才)项目(QNBJ202329);安徽省重点研究与开发计划资助项目(2022f04020003);安徽省质量基础设施标准化专项资助项目(2022MKSO4)。

摘　　要：目的:开发一种基于金铂纳米花的增敏型侧向免疫层析试纸。方法:通过优化金铂纳米花的合成条件及侧向免疫层析试纸体系参数,构建新型免疫层析试纸,并进行灵敏度验证。结果:基于金铂纳米花的侧向免疫层析试纸对兔免疫球蛋白G(Immunog Iobu Iin G,Tg G)的检测限为0.05 ng·mL^(-1),较传统基于金纳米粒子的侧向免疫层析试纸低。结论:金铂纳米花为侧向免疫层析试纸提供了新标记物,并提高了灵敏度,在免疫测定及其他生物分析领域展现出广阔的应用前景。Objective:To develop a sensitized lateral immunochromatographic test strip based on gold platinum nanoflowers.Method:By optimizing the synthesis conditions of gold platinum nanoflowers and the parameters of the lateral immunochromatographic test strip system,a novel immunochromatographic test strip was constructed and its sensitivity was verified.Result:The detection limit of rabbit immunogIobuIin G(IgG)using the lateral immunochromatographic test strip based on gold platinum nanoflowers was 0.05 ng·mL^(-1),which was lower than the traditional lateral immunochromatographic test strip based on gold nanoparticles.Conclusion:Gold platinum nanoflowers provide a new marker for lateral immunochromatographic test strips and improve sensitivity,showing broad application prospects in immunoassay and other biological analysis fields.

关 键 词：金纳米粒子 铂 酶模拟物 纳米花 侧向免疫分析试纸 

分 类 号：R917[医药卫生—药物分析学]