HGF调控葡萄膜黑色素瘤细胞迁移的分子机制研究  

Molecular Mechanism of HGF Mediating Cell Migration in Uveal Melanoma

作　　者：朱鲜 郑丹丹 侯强[1] ZHU Xian;ZHENG Dandan;HOU Qiang(School of Ophthalmology and Optometry,Eye Hospital,Wenzhou Medical University,Wenzhou 325000,China)

机构地区：[1]温州医科大学眼视光学院/附属眼视光医院,温州325000

出　　处：《中国细胞生物学学报》2025年第4期851-857,共7页Chinese Journal of Cell Biology

基　　金：浙江省自然科学基金探索项目(批准号:LY21H120006)资助的课题。

摘　　要：肝细胞生长因子(hepatocyte growth factor, HGF)对葡萄膜黑色素瘤(uveal melanoma,UM)的进展发挥着重要作用,该文旨在研究HGF调控UM细胞迁移的分子机制。通过基因芯片分析,筛选了一系列受HGF调控且与迁移相关的基因;定量PCR、Western blot实验验证基因芯片结果,发现HGF抑制了神经纤维瘤蛋白(NF2)和金属蛋白酶3组织抑制剂(TIMP3)基因编码的mRNA和蛋白水平;使用siRNA转染UM细胞,干扰候选基因NF2和TIMP3的表达,检测其对UM细胞迁移能力的影响,细胞迁移实验发现了下调NF2和TIMP3基因的表达增强了UM细胞的迁移能力。这些结果说明HGF通过影响NF2和TIMP3基因编码的蛋白表达来介导UM细胞的迁移。HGF(hepatocyte growth factor)plays an important role in the progression of UM(uveal mela-noma).This study aimed to investigate the molecular mechanism by which HGF regulates UM cell migration.A series of migration-related genes regulated by HGF were screened by microarray analysis.Quantitative PCR and Western blot experiments verified the results of gene microarray,and it was found that HGF inhibited the levels of mRNAs and proteins encoded by NF2(neurofibromatoprotein)and TIMP3(tissue inhibitor of metalloproteinase 3)genes.UM cells were transfected with siRNA to interfere with the expression of candidate genes NF2 and TIMP3,and their effects on the migration ability of UM cells were detected.Cell migration experiments showed that down-regulating the expression of NF2 and TIMP3 genes enhanced the migration ability of UM cells.These results suggest that HGF mediates UM cell migration by influencing the expression of proteins encoded by the NF2 and TIMP3 genes.

关 键 词：葡萄膜黑色素瘤 HGF 迁移 NF2 TIMP3 

分 类 号：R739.7[医药卫生—肿瘤]