基于全基因组测序对内蒙古鄂尔多斯市布鲁菌种型的鉴定  

Identification of Brucella strains in Ordos City,Inner Mongolia based on whole genome sequencing

作　　者：王娜娜 贾媛 王昇 肖洪涛 杨春燕[2] 颜璐 李鹏 靳尚武[2] WANG Nana;JIA Yuan;WANG Sheng;XIAO Hongtao;YANG Chunyan;YAN Lu;LI Peng;JIN Shangwu(School of Public Health,Inner Mongolia Medical University,Hohhot,Inner Mongolia 010000,China;Ordos Center for Disease Control and Prevention,Ordos,Inner Mongolia 017000,China;Ordos No.2 Hospital,Ordos,Inner Mongolia 017000,China)

机构地区：[1]内蒙古医科大学公共卫生学院,内蒙古呼和浩特010000 [2]鄂尔多斯市疾病预防控制中心,内蒙古鄂尔多斯017000 [3]鄂尔多斯市二医院,内蒙古鄂尔多斯017000

出　　处：《中国热带医学》2025年第3期333-338,共6页China Tropical Medicine

基　　金：鄂尔多斯市科技计划项目(No.2021YY社213-68)。

摘　　要：目的 探索内蒙古鄂尔多斯市布鲁菌的全基因组结构,进行生物信息学分析,探讨多种布鲁菌种型鉴定方法,为鄂尔多斯市布鲁菌的研究提供理论基础。方法 采用illumina Miseq基因测序仪进行全基因组测序,运用Microobench病原微生物基因分析工作站对15株布鲁菌进行基因组组分分析、基因组注释分析、16Sr DNA分析、SNP位点和插入缺失变异分析、MLST位点分析、系统进化树分析。结果 15株布鲁菌测序平均深度170.04×,基因组平均大小为3 290 725 bp,基因组覆盖度为99.93%,组装文件GC含量平均值为54.69%。16Sr DNA分析结果显示15株布鲁菌与马耳他布鲁菌同源性为100%。单核苷酸突变位点和插入缺失变异分析结果显示15株布鲁菌单核苷酸突变数平均数为2 412个,多核苷酸突变数平均数为39个,插入变异数平均数为131个,缺失变异数平均数为153个,复合型变异数平均数为43个,变异总数平均数为2 778个。MLST位点分析结果显示15株布鲁菌均为ST-8型。结论 本研究基于对鄂尔多斯市15株布鲁菌的全基因组测序进行了多种种型鉴定方法,不同方法各有侧重,需要多种方法互相印证,从而获得更为准确的实验结果,为鄂尔多斯市布鲁菌的研究提供更多研究基础,为鄂尔多斯市布鲁菌病的防控提供更多数据和理论支撑。Objective To explore the whole genome structure of Brucella in Ordos city,conduct bioinformatics analysis,and explore a variety of Brucella species type identification methods,so as to provide a theoretical basis for the study of Brucella in Ordos City.Methods The whole genome was sequenced using illumina Miseq gene sequencer.The Microobench microbial gene analysis workstation was used to analyze the genome components,genome annotation,16Sr DNA,SNP sites,insertions and deletions,MLST sites,and phylogenetic tree of 15 strains of Brucella.Results The average sequencing depth of 15 Brucella strains was 170.04×,the average genome size was 3290725 bp,the genome coverage was 99.93%,and the average GC content of assembly files was 54.69%.The results of 16Sr DNA analysis showed that the homology of 15 Brucella strains and Brucella Maltese was 100%.The analysis results of SNP sites and insert-deletion variation showed that the average number of SNPs of the 15 strains of Brucella was 2412,the average number of polynucleotide mutations was 39,the average number of insertional variants was 131,the average number of missing variants was 153,the average number of complex variants was 43,and the average number of total variants was 2778.MLST locus analysis showed that all 15 strains of Brucella were ST-8.Conclusion Based on the whole genome sequencing of 15 strains of Brucella in Ordos City,this study carried out a variety of type identification methods.Different methods have different focuses,and multiple methods are needed to confirm each other,so as to obtain more accurate experimental results and provide more research basis for the study of Brucella,to provide more data and theoretical support for the control and prevention of brucellosis in Ordos City.

关 键 词：布鲁菌 全基因组测序 种型鉴定 鄂尔多斯 

分 类 号：R378.5[医药卫生—病原生物学]