C57BL/6J小鼠视敏度发育不同检测方法的比较研究  

Evaluation and comparison of visual acuity measurement methods in developing C57BL/6J mice

作　　者：王雪纯 刘新龙 郭雅图 张伟[1] Wang Xuechun;Liu Xinlong;Guo Yatu;Zhang Wei(Clinical College of Ophthalmology of Tianjin Medical University,Tianjin Eye Hospital,Nankai University Affiliated Eye Hospital,Tianjin Eye Institute,Tianjin Key Laboratory of Ophthalmology and Visual Science,Tianjin 300020,China)

机构地区：[1]天津医科大学眼科临床学院,天津市眼科医院,南开大学附属眼科医院,天津市眼科研究所,天津市眼科学与视觉科学重点实验室,天津300020

出　　处：《中华眼科杂志》2025年第4期279-286,共8页Chinese Journal of Ophthalmology

基　　金：天津市医学重点学科(专科)建设项目(TJYXZDXK-016A);天津市卫生健康科技面上项目(TJWJ2021MS042);南开大学视光科学研究院开放课题(NKSGY202301)。

摘　　要：目的探讨C57BL/6J小鼠视敏度的发育特点及不同检测方法的一致性和可靠性。方法实验研究。将172只C57BL/6J小鼠按照天龄(P)13或14(睁眼第1天)、15、16、18、21(视觉发育关键期开始)、28(视觉发育关键期高峰)、35(视觉发育关键期结束)、60 d(成年)分为8个组(分别为18、11、10、21、29、28、25、30只小鼠),采用视觉电生理、视觉反射任务和视觉水箱任务(VWT)3种方法分别检测视敏度,并进行比较。采用最小显著性差异t检验、单因素方差分析和独立样本t检验进行统计学分析。结果视觉电生理检测单眼视敏度,P13/14组为(0.20±0.06)周/度(cpd),P21组为(0.44±0.08)cpd,P35组为(0.52±0.06)cpd,组间比较的差异均有统计学意义(均P<0.05);P60组为(0.56±0.05)cpd,与P35组比较,差异无统计学意义(P>0.05)。视觉反射任务方法检测双眼视敏度,P13/14组为(0.11±0.06)cpd,P15组为(0.20±0.07)cpd,P16组为(0.35±0.05)cpd,P18组为(0.40±0.05)cpd,P21组为(0.47±0.05)cpd,组间比较的差异均有统计学意义(均P<0.05);P60组为(0.50±0.05)cpd,与P21组比较,差异无统计学意义(P>0.05)。采用视觉水箱任务方法检测P60组双眼视敏度为(0.50±0.03)cpd,3种方法检测P60组的视敏度比较,差异无统计学意义(P>0.05)。结论C57BL/6J小鼠从睁眼第1天至视觉发育关键期开始视敏度迅速增长,然后减缓增长至成年水平。3种方法检测成年C57BL/6J小鼠的视敏度为0.50~0.56 cpd,结果相近,方法可靠。Objective To investigate the developmental characteristics of visual acuity in C57BL/6J mice and to evaluate the consistency and reliability of different measurement methods.Methods This was an experimental study.One hundred and seventy-two C57BL/6J mice were divided into eight groups based on postnatal days(P):P13/14(first day of eye opening,18 mice),P15(11 mice),P16(10 mice),P18(21 mice),P21(onset of the critical period for visual development,29 mice),P28(peak of the critical period,28 mice),P35(end of the critical period,25 mice),and P60(adulthood,30 mice).Visual acuity was assessed using three methods:visual evoked potentials(VEP),the optomotor reflex(OMR)task,and the visual water task(VWT).Statistical analyses were conducted using the least significant difference t-test,one-way ANOVA,and independent sample t-tests.Results VEP measurements of monocular visual acuity showed that the visual acuity in the P13/14 group was(0.20±0.06)cycles per degree(cpd),and increased to(0.44±0.08)cpd in the P21 group and(0.52±0.06)cpd in the P35 group,with significant differences between groups(all P<0.05).The visual acuity in the P60 group was(0.56±0.05)cpd,which was not significantly different from that in the P35 group(P>0.05).For binocular visual acuity assessed by the OMR task,the P13/14 group had a visual acuity of(0.11±0.06)cpd,which increased to(0.20±0.07)cpd in the P15 group,(0.35±0.05)cpd in the P16 group,(0.40±0.05)cpd in the P18 group,and(0.47±0.05)cpd in the P21 group,with significant differences between groups(all P<0.05).The visual acuity in the P60 group was(0.50±0.05)cpd,which was not significantly different from that in the P21 group(P>0.05).The binocular visual acuity assessed by the VWT in the P60 group was(0.50±0.03)cpd.There was no significant difference in visual acuity measured using the three methods in the P60 group(P>0.05).Conclusions Visual acuity in C57BL/6J mice increased rapidly from the first day of eye opening to the onset of the critical period for visual development,followed by a sl

关 键 词：小鼠 近交C57BL 视敏度 诱发电位 视觉 行为 动物 迷宫学习 光刺激 评价研究(主题) 

分 类 号：R774.6[医药卫生—眼科]