顶空固相微萃取-气相色谱法检测野生菌中甲醛的技术研究  

Study on determination of formaldehyde in wild mushroom by headspace solid phase microextraction-gas chromatography method

作　　者：赵丽[1] 张瑞雨[1] 左琪琦 段毅宏[3] 李彦生[3] 刘建辉[3] ZHAO Li;ZHANG Ruiyu;ZUO Qiqi;DUAN Yihong;LI Yansheng;LIU Jianhui(Physico-Chemical Laboratory,Kunming Center for Disease Control and Prevention,Kunming,Yunnan 650228,China;School of Public Health,Kunming Medical University,Kunming,Yunnan 650500,China;Testing Center,Yunnan Center for Disease Control and Prevention,Kunming,Yunnan 650032,China)

机构地区：[1]昆明市疾病预防控制中心理化检验科,云南昆明650228 [2]昆明医科大学公共卫生学院,云南昆明650500 [3]云南省疾病预防控制中心检测中心,云南昆明650032

出　　处：《职业与健康》2025年第5期607-610,616,共5页Occupation and Health

基　　金：昆明市卫生科技人才培养项目(2021-SW(省)-30);昆明市卫生科研项目(2023-12-06-002)。

摘　　要：目的建立顶空固相微萃取-气相色谱法快速测定野生菌中甲醛的方法。方法以顶空固相微萃取(headspace solid phase microextraction,HS-SPME)进行前处理,采用气相色谱-氢火焰离子检测器(gas chromatography-hydrogen flame ion detector,GC-FID)测定。通过比较不同的检测方法,优化萃取纤维涂层种类、平衡时间、萃取时间、萃取温度以及解析温度等影响萃取效率的因素,获得较为理想的检测方法。结果在给定的条件下,方法线性范围为0.01~4.00 mg/kg,相关系数为0.998~0.999,加标回收率为99.6%~109.7%,相对标准偏差(RSDs)为4.78%~8.24%,在11个野生菌样品中检出甲醛,其浓度范围为0.011~0.293 mg/kg。结论所建立的方法操作简单,耗时短,灵敏度高,适用于大批量野生菌中甲醛的快速检测。Objective To establish a method for rapid determination of formaldehyde in wild mushrooms by headspace solid phase microextraction-gas chromatography(HS-SPME-GC).Methods The headspace solid phase microextraction(HS-SPME)was used for the pre-treatment,and gas chromatography-hydrogen flame ion detector(GC-FID)was used for the determination.By comparing different detection methods and optimizing the factors affecting the extraction efficiency such as the extraction fiber coating type,equilibrium time,extraction time,extraction temperature and resolving temperature,a more ideal detection method was obtained.Results Under given conditions,the linear range of the method was 0.01-4.00 mg/kg,the correlation coefficient was 0.998-0.999,the average standard recoveries were 99.6%-109.7%,and the relative standard deviations(RSDs)was 4.78%-8.24%.The formaldehyde was detected in 11 wild mushroom samples,with the concentration range of 0.011-0.293 mg/kg.Conclusion The method is simple in operation,short in time and high in sensitivity,and is suitable for rapid detection of formaldehyde in large quantities of wild mushrooms.

关 键 词：顶空固相微萃取 气相色谱 野生菌 甲醛 

分 类 号：R115[医药卫生—公共卫生与预防医学]