淋巴细胞染色体畸变生物剂量估算实验室间能力评价方法探讨  

Exploration of inter laboratory capacity evaluation method for biological dose estimation of lymphocyte chromosome aberration

作　　者：梅丹 王芳[2] 王静[1] 罗伟[1] MEI Dan;WANG Fang;WANG Jing;LUO Wei(Guardianship Department,Health Supervision Institute,Hubei Provincial Center for Disease Control and Prevention,Wuhan,Hubei 430079,China;Department of Laboratory,Wuhan University Hospital,Wuhan,Hubei 430072,China)

机构地区：[1]湖北省疾病预防控制中心卫监所监护部,湖北,武汉430079 [2]武汉大学医院检验科,湖北武汉430072

出　　处：《职业与健康》2025年第5期622-625,共4页Occupation and Health

摘　　要：目的探讨外周血淋巴细胞染色体畸变生物剂量估算能力评价方法,客观评价实验室检测能力,促进提升检测水平。方法以2023年全国参评实验室上报数据为依据,分析现有评价方法存在的问题,研究改进能客观评价实验室检测能力的方法。结果按照射剂量1.6、2.7、3.7 Gy分组,用Z分数值评价的合格率只有A类3.7 Gy组合格率低于偏差评价的合格率,其他组均高于偏差评价的合格率。A类2.7 Gy组、3.7 Gy组2种方法评价的合格率差异均无统计学意义(均P>0.05),其他组合格率比较,差异均有统计学意义(均P<0.05)。各组上报估算剂量均值均低于照射剂量,为负偏差,除B类1.6 Gy组相对照射剂量偏差较小(-1.88%)外,其他组偏差在-11.48%~-20.54%,且变异水平在12.63%~20.81%。使用自建曲线的实验室估算均值略高于国标推荐曲线的实验室,但差异无统计学意义(P>0.05)。用各组的估算均值作为靶值,用概率t确定允许误差范围可以便捷的评估参评实验室检测能力。结论现有评价方法没有将该项检测的特点与参评实验室实际情况统一考虑,在确定靶值及允许误差范围方面需结合具体情况逐步完善。Objective To explore the evaluation method for estimating the biological dose of chromosomal aberrations in peripheral blood lymphocytes,objectively evaluate the laboratory testing ability,and promote the improvement of testing level.Methods Based on the data submitted by the national participating laboratories in 2023,analyze the problems of existing evaluation methods,and study and improve methods that can objectively evaluate laboratory testing capabilities.Results According to the radiation doses of 1.6,2.7,and 3.7 Gy,the Z-score evaluation method showed that the pass rate of the A categories 3.7 Gy group was lower than that of the deviation evaluation method,while the pass rates of other groups were higher than that of the deviation evaluation method.There were no statistically significant difference in the pass rate between the two evaluation methods in A categories 2.7 Gy group and 3.7 Gy group(both P>0.05),while there was statistically significant difference in other groups(all P<0.05).The average estimated dose reported by each group was lower than the irradiation dose,indicating a negative deviation.Except for the B categories 1.6 Gy group,which had a relatively small relative irradiation dose deviation(-1.88%),the deviation of the other groups ranged from-11.48%to-20.54%,and the variation level ranged from 12.63%to 20.81%.The estimated average value of laboratories using self-constructed curves was slightly higher than that of laboratories using national standard recommended curves,but the difference was not statistically significant(P>0.05).Using the estimated mean value of each group as the target value and using the probability t to determine the allowable error range can conveniently evaluate the testing ability of the participating laboratories.Conclusion The existing evaluation methods have not consider the characteristics of this test and the actual situation of participating laboratories uniformly,and needs to be gradually improved in terms of determining target values and allowable error range

关 键 词：染色体畸变 室间能力评价 实验室 检测 方法 

分 类 号：R144[医药卫生—公共卫生与预防医学]