Chitosan oligosaccharide enhances the anti-cancer effects of 5-fluorouracil on SNU-C5 colorectal cancer cells by activating ERK  

作　　者：JI-SU HAN HYE-JIN BOO JIN WON HYUN HEESANG SONG IN-YOUB CHANG SANG-PIL YOON 

机构地区：[1]Jeju Research Center for Natural Medicine,Jeju National University,Jeju,63243,Republic of Korea [2]Department of Biochemistry and Molecular Biology,Chosun University School of Medicine,Gwangju,61452,Republic of Korea [3]Department of Anatomy,Chosun University School of Medicine,Gwangju,61452,Republic of Korea [4]Department of Anatomy,College of Medicine,Jeju National University,Jeju,63243,Republic of Korea

出　　处：《Oncology Research》2025年第4期873-884,共12页肿瘤学研究(英文)

基　　金：supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Korea government(2021R1F1A1063023);by the Ministry of Education(RS-2023-00270936).

摘　　要：Background:Chitosan oligosaccharide(COS)is the major degradation product of chitosan by enzymatic processes.COS,with complete water solubility,exerts significant biological effects,including anti-cancer activity.We investigated the anti-tumor effects of COS on colorectal cancer as effective therapeutic methods with low side effects are lacking.Methods:COS was obtained from low molecular weight chitosan by an enzymatic method and the anticancer effects were measured by cell viability assay,flow cytometry analysis,Western blotting,and xenograft.Results:COS suppressed the proliferation of SNU-C5 cells compared to other colorectal cancer cells,but higher concentrations were required in the xenograft model.Co-treatment with 5-fluorouracil(5-FU)and COS enhanced the anti-cancer effects of 5-FU in SNU-C5 cells in vitro and in vivo.Flow cytometry revealed that COS induced cell cycle arrest at the G0/G1 phase without 5-FU or at the S and G2/M phases with 5-FU but did not affect cell death pathways.COS increased extracellular signal-regulated protein kinase(ERK)activation with or without 5-FU,whereas 5-FU treatment increased p53 activation.A low-dose of an ERK inhibitor suppressed COS-induced ERK activation and resulted in higher proliferation compared with COS.Conclusions:These results suggest that COS might enhance the anti-cancer effects of 5-FU in SNU-C5 colorectal cancer cells by activating ERK.

关 键 词：Chitosan oligosaccharide(COS) Colorectal cancer(CRC) 5-Fluorouracil(5-FU) ERK(Extracellular signal-regulated protein kinase) 

分 类 号：R735.3[医药卫生—肿瘤]