2型糖尿病视网膜病变患者血清Notch1、Notch3和JAG1水平变化及检测意义  

Changes and detection significance of serum Notch1,Notch3 and JAG1 levels in patients with type 2 diabetic retinopathy

作　　者：高珊[1] 乔媛[1] 董春萍[1] GAO Shan;QIAO Yuan;DONG Chunping(Department of Endocrinology,Shaanxi Provincial People’s Hospital,Xi’an 710068,China)

机构地区：[1]陕西省人民医院内分泌科,陕西西安710068

出　　处：《陕西医学杂志》2025年第5期688-692,共5页Shaanxi Medical Journal

基　　金：陕西省重点研发计划项目(2019SF-971)。

摘　　要：目的:探讨2型糖尿病视网膜病变(DR)患者血清Notch1、Notch3和JAG1水平变化及检测意义。方法:选取2型糖尿病(T2DM)患者147例,根据有无并发DR将患者分为病变组(45例)和未病变组(102例)。收集两组患者临床资料,采用实时荧光定量PCR(RT-qPCR)检测血清Notch1、Notch3和JAG1表达水平。采用Spearman法分析血清Notch1、Notch3和JAG1表达水平与T2DM患者并发DR的相关性。绘制受试者工作特征(ROC)曲线分析血清Notch1、Notch3和JAG1对T2DM患者并发DR的预测价值。采用多因素Logistic回归模型分析T2DM患者并发DR的影响因素。结果:病变组患者T2DM病程、糖化血红蛋白(HbAlc)水平高于未病变组(均P<0.05)。与未病变组比较,病变组患者血清Notch1、Notch3和JAG1表达水平降低(均P<0.05)。血清Notch1、Notch3和JAG1表达水平与T2DM患者并发DR呈负相关(均P<0.05)。血清Notch1、Notch3和JAG1联合预测T2DM患者并发DR的曲线下面积(AUC)为0.905高于三者单独预测的AUC(均P<0.05)。T2DM病程、HbAlc、血清Notch1、Notch3和JAG1为T2DM患者并发DR的独立影响因素(均P<0.05)。结论:T2DM并发DR患者血清Notch1、Notch3和JAG1表达水平降低,对T2DM患者并发DR有一定预测价值,且三者联合预测的效能更高。Objective:To investigate the changes in serum Notch1,Notch3 and JAG1 levels and the significance of detection in patients with type 2 diabetic retinopathy(DR).Methods:A total of 147 patients with type 2 diabetes mellitus(T2DM)were selected and divided into two groups:the DR group(45 cases)and the non-DR group(102 cases).Clinical data were collected from both groups.The expression levels of serum Notch1,Notch3 and JAG1 were detected using real-time quantitative PCR(RT-qPCR).Spearman correlation analysis was used to evaluate the correlation between the expression levels of serum Notch1,Notch3 and JAG1 and the occurrence of DR in T2DM patients.Receiver operating characteristic(ROC)curves were plotted to analyze the predictive value of serum Notch1,Notch3 and JAG1 for DR in T2DM patients.Multivariate logistic regression analysis was performed to identify the influencing factors of DR in T2DM patients.Results:The duration of T2DM and the level of HbA1c were higher in the DR group than in the non-DR group(all P<0.05).Compared with the non-DR group,the expression levels of serum Notch1,Notch3 and JAG1 were significantly lower in the DR group(all P<0.05).The expression levels of serum Notch1,Notch3 and JAG1 were negatively correlated with the occurrence of DR in T2DM patients(all P<0.05).The AUC for the combined prediction of DR by serum Notch1,Notch3 and JAG1 was 0.905,which was higher than that of individual predictions(all P<0.05).The duration of T2DM,HbA1c and serum levels of Notch1,Notch3 and JAG1 were identified as independent influencing factors for DR in T2DM patients(all P<0.05).Conclusion:The expression levels of serum Notch1,Notch3 and JAG1 are decreased in T2DM patients with DR,and they have predictive value for DR in T2DM patients.The combined prediction of these three markers provides higher efficacy.

关 键 词：2型糖尿病 糖尿病视网膜病变 NOTCH1 NOTCH3 JAG1 预测价值 

分 类 号：R587.1[医药卫生—内分泌]