基于Wnt1和GSK-3β探究金郁欢对抑郁模型小鼠的抗抑郁机制研究  

Exploration of the antidepressant mechanism of Jin Yuhuan in depression model mice based on Wnt1 and GSK-3β

作　　者：张梦迪 张雨薇[1] 仲丽丽[2] 代巧妹[1] 杨婧[1] 李冀[1] 刘宏[1] ZHANG Mengdi;ZHANG Yuwei;ZHONG Lili;DAI Qiaomei;YANG Jing;LI Ji;LIU Hong(School of Basic Medical Sciences,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Pathology the First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学基础医学院,黑龙江哈尔滨150040 [2]黑龙江中医药大学第一附属医院病理科,黑龙江哈尔滨150040

出　　处：《陕西中医》2025年第5期579-584,共6页Shaanxi Journal of Traditional Chinese Medicine

基　　金：黑龙江省博士后基金资助项目(LBH-Z15207);黑龙江省哈尔滨市科技局青年后备人才计划项目(2016RAQXJ202)。

摘　　要：目的:采用慢性温和不可预知应激法制作小鼠抑郁模型,探究金郁欢治疗抑郁小鼠所涉及的相关机制。方法:将60只ICR小鼠进行随机分配,设立以下五个组别:对照组、模型组、低剂量金郁欢处理组、高剂量金郁欢处理组以及氟西汀治疗组,每组12只。悬尾实验观察动物抑郁样行为;尼氏染色检测小鼠海马CA3区尼氏小体平均黑度;免疫组织化学法、Western blotting检测Wnt1和GSK-3β蛋白水平;RT-PCR检测Wnt1和GSK-3βmRNA表达情况。结果:相较于对照组,模型组小鼠在悬尾测试中的静止持续时间出现了明显的延长,尼氏小体平均黑度值降低,Wnt1、Wnt1 mRNA表达减少,GSK-3β、GSK-3βmRNA表达增多。与模型组相比,金郁欢组悬尾静止时间缩短,尼氏小体平均黑度值升高,Wnt1、Wnt1 mRNA表达增多,GSK-3β、GSK-3βmRNA表达减少。结论:金郁欢可有效改善抑郁症小鼠抑郁样行为,通过改变Wnt1、GSK-3β蛋白表达,发挥抗抑郁作用。Objective:A chronic mild unpredictable stress method was used to create a mouse model of depression and exploring the relevant mechanisms involved in the treatment of depressed mouse with Jin Yuhuan(JYH).Methods:Sixty ICR mice were randomly divided into control group,model group,JYH low-dose group,JYH high-dose group,and fluoxetine group,12 mice in each group.Tail suspension test was used to observe the depression-like behavior of the animals;Nissl staining was used to detect the average blackness of Nissl body in the CA3 region of the mice hippocampus.Immunohistochemistry and Western blotting were used to detect the protein levels of Wnt1 and GSK-3β;and RT-PCR was used to detect the expression of Wnt1 and GSK-3βmRNA.Results:Compared with the control group,mice in the model group had a significantly increased tail suspension test time,a decreased average blackness value of Nissl body,a decreased expression of Wnt1 and Wnt1 mRNA,and an increased expression of GSK-3βand GSK-3βmRNA.Compared with the model group,the tail suspension time was shortened in the JYH group,the average blackness value of Nissl body was increased,the expression of Wnt1,Wnt1 mRNA was increased,and the expression of GSK-3β,GSK-3βmRNA was decreased.Conclusion:JYH can effectively improve depressive-like behavior in depressed mice and exert antidepressant effects by altering Wnt1 and GSK-3βprotein expression.

关 键 词：抑郁症 WNT1 GSK-3Β 乌腺金丝桃 郁金 合欢花 

分 类 号：R749.2[医药卫生—神经病学与精神病学]