Exploring the Efficacy of BMSC Transplantation via Various Pathways for Treating Cholestatic Liver Fibrosis in Mice  

作　　者：Junjie Ren Zixu Li Xinrui Shi Tingting Lyu Xiaonan Li Min Ge Qizhi Shuai Tingjuan Huang 

机构地区：[1]Department of Gastroenterology and Hepatology,The First Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi,China [2]Department of Academy of Medical Sciences,Shanxi Medical University,Taiyuan 030001,Shanxi,China [3]Liver Research Center,Beijing Friendship Hospital,Capital Medical University,Beijing Key Laboratory of Translational Medicine on Liver Cirrhosis,National Clinical Research Center for Digestive Disease,Beijing 100050,China [4]Department of Cancer Radiotherapy,Fifth Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi,China [5]School of Clinical Medicine,Medical College,Hebei University of Engineering,Handan 056038,Hebei,China [6]Department of Biochemistry and Molecular Biology,Shanxi Key Laboratory of Birth Defect and Cell Regeneration,MOE Key Laboratory of Coal Environmental Pathogenicity and Prevention,Shanxi Medical University,Taiyuan 030001,Shanxi,China [7]Department of Hepatopancreatobiliary Surgery,First Hospital of Shanxi Medical University Key Laboratory of Birth Defect and Cell Regeneration,Shanxi Medical University,Taiyuan 030001,Shanxi,China

出　　处：《Biomedical and Environmental Sciences》2025年第4期447-458,共12页生物医学与环境科学(英文版)

基　　金：supported by grants from the Natural Science Research Project of Shanxi Basic Research Program [20210302123246];the Scientific Research Project of Shanxi Provincial Health Commission [2020079];the Natural Science Foundation Youth Fund of Hebei Province[C2022402032];the Shanxi Province Higher Education “Billion Project” Science and Technology Guidance Project [BYJL036]。

摘　　要：Objective To compare the therapeutic efficacy of portal and tail vein transplantation of bone marrowderived mesenchymal stem cells(BMSCs) against cholestatic liver fibrosis in mice.Methods BMSCs were isolated and co-cultured with starvation-activated hepatic stellate cells(HSCs).HSC activation markers were identified using immunofluorescence and qRT-PCR. BMSCs were injected into the liver tissues of bile duct ligation(BDL) mice via the tail and portal veins. Histomorphology, liver function, inflammatory cytokines, and the expression of key proteins were all determined in the liver tissues.Results BMSCs inhibited HSC activation by reducing α-SMA and collagen I expression. Compared to tail vein injection, DIL-labeled BMSCs injected through the portal vein maintained a high homing rate in the liver. Moreover, BMSCs transplanted through the portal vein resulted in greater improvement in liver color, hardness, and gallbladder size than did those transplanted through the tail vein. Furthermore,BMSCs injected by portal vein, but not tail vein, markedly ameliorated liver function, reduced the secretion of inflammatory cytokines, including TNF-α, IL-6, and IL-1β, and decreased α-SMA + hepatic stellate cell(HSC) activation and collagen fiber formation.Conclusion The therapeutic effect of BMSCs on cholestatic liver fibrosis in mice via portal vein transplantation was superior to that of tail vein transplantation. This comparative study provides reference information for further BMSC studies focused on clinical cholestatic liver diseases.

关 键 词：Bone marrow mesenchymal stem cells Portal vein Tail vein Cholestatic hepatic fibrosis Administration route 

分 类 号：R575.2[医药卫生—消化系统]