铁基金属有机骨架对中华青鳉毒性作用的持续性与恢复性  

Persistence and Recovery of Toxicity Induced by Iron-Based Metal-Organic Framework to Chinese Medaka(Oryzias sinensis)

作　　者：叶君玉 李衍红 李以玲 高阳 夏斌[1] 崔正国[1] 曲克明[1] YE Junyu;LI Yanhong;LI Yiling;GAO Yang;XIA Bin;CUI Zhengguo;QU Keming(State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Faculty of Fisheries,Zhejiang Ocean University,Zhoushan 316022,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]海水养殖生物育种与可持续产出全国重点实验室,中国水产科学研究院黄海水产研究所,青岛266071 [2]浙江海洋大学水产学院,舟山316022 [3]大连海洋大学水产与生命学院,大连116023

出　　处：《生态毒理学报》2025年第2期152-160,共9页Asian Journal of Ecotoxicology

基　　金：山东省自然科学基金海外优青项目(2023HWYQ-103);山东省泰山学者专项(tsqn202306296);中国水产科学研究院基本科研业务费资助项目(20603022024017)。

摘　　要：铁基金属有机骨架(Fe-MOFs)在环境治理和修复领域展现出广泛的应用前景。然而,在Fe-MOFs合成、应用及处置等过程中,不可避免地会释放到水环境中,对生态系统和生物体造成潜在毒害作用。目前,关于Fe-MOFs生态毒性效应的研究仍较为有限。以中华青鳉(Oryzias sinensis)作为实验对象,探究了一种典型Fe-MOF(MIL-88B(Fe))对中华青鳉的毒性持久性和恢复性效应。实验将中华青鳉在0.4 mg·L^(-1)的Fe-MOFs中胁迫14 d,随后进行14 d恢复阶段的观察。转录组学分析表明,在暴露阶段,Fe-MOFs显著影响了鱼体内核糖体功能、碳代谢、内质网蛋白质加工、糖酵解/糖异生以及丙酮酸代谢等关键生物学过程。在恢复阶段,神经活性配体-受体相互作用、cAMP信号通路、不饱和脂肪酸的生物合成和谷氨酸突触的破坏与Fe-MOFs诱导的毒性持久性密切相关。这些发现有助于揭示Fe-MOFs在鱼体内毒性效应的持续性及其恢复机制,对评估其生态环境风险具有重要的意义。Iron-based metal-organic frameworks(Fe-MOFs)have garnered significant attention for their diverse applications in environmental fields.However,the increasing applications of Fe-MOFs raise concerns about their potential risks to living organisms.Nevertheless,the persistence and recovery of their toxicity remains largely unknown.Herein,two sub-experiments(exposure and recovery experiments)were conducted to reveal the persistence of the toxicity of a representative Fe-MOF,MIL-88B(Fe),to Chinese medaka(Oryzias sinensis).Oryzias sinensis were exposed to 0.4 mg·L^(-1)Fe-MOFs for 14 days.Transcriptome sequencing was employed to investigate the molecular responses of Oryzias sinensis during Fe-MOFs exposure and after recovery.During the exposure stage,Fe-MOFs significantly altered ribosome,carbon metabolism,protein processing in endoplasmic reticulum,glycolysis/gluconeogenesis,and pyruvate metabolism at exposure stage.In the recovery stage,disruption of neuroactive l igand-receptor interaction,cAMP signaling pathway,biosynthesis of unsaturated fatty acids,and glutamatergic s ynapse was linked to the persistence of Fe-MOFs induced toxicity.These findings highlight the mechanisms u nderlying the persistence of Fe-MOFs induced toxicity and provide critical insights for the accurate ecological risk assessment of Fe-MOFs.

关 键 词：铁基金属有机骨架 中华青鳉 生态毒性 转录组学 恢复 持久性 

分 类 号：X171.5[环境科学与工程—环境科学]