摄食暴露富砷贝类后小鼠体内砷形态化合物的代谢分布  

Metabolic Distribution of Arsenic Species Compounds in Mice after Ingestion of Exposed Arsenic-rich Shellfish

作　　者：冉茂霞 莫晓 张晓莹 史永富[1] 叶洪丽 黄冬梅[1] 李思曼 方长玲 RAN Maoxia;MO Xiao;ZHANG Xiaoying;SHI Yongfu;YE Hongli;HUANG Dongmei;LI Siman;FANG Changling(Key Laboratory of Oceanic and Polar Fisheries,Ministry of Agriculture and Rural Affairs,China East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;College of Food Science and Engineering,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]中国水产科学研究院东海水产研究所,农业农村部远洋与极地渔业创新重点实验室,上海200090 [2]上海海洋大学食品学院,上海201306 [3]大连海洋大学食品科学与工程学院,大连116023

出　　处：《生态毒理学报》2025年第2期204-212,共9页Asian Journal of Ecotoxicology

基　　金：中国水产科学研究院东海水产研究所基本科研业务费资助项目(2021M05)。

摘　　要：通过连续14 d的小鼠摄食贝类暴露实验以获得砷在生物体中的累积、代谢、转化情况。结果表明膳食暴露14 d后,小鼠摄入的总砷最终约70%以粪便形式排出。总砷在各脏器/组织中的蓄积为胃(0.26~0.54μg·g^(-1))>肠(0.12~0.42μg·g^(-1))>脾(0.031~0.162μg·g^(-1))>肾(0.06~0.12μg·g^(-1))>肝脏(0.049~0.091μg·g^(-1)),在其余脏器/组织中的蓄积不明显(0.016~0.065μg·g^(-1));食物中的砷甜菜碱(AsB)在暴露14 d后约有46%以粪便形式排出,而二甲基砷(DMA)约有576%排出,DMA排出的量远远高于从食物中摄入的量,表明小鼠体内发生了砷的甲基化反应;在各脏器/组织中,仅在肠道中检出砷酸盐(AsⅤ),而蓄积含量(0.0054~0.0116μg·g^(-1))很低,在其余脏器/组织中的蓄积主要以砷胆碱(AsC)(0.0011~0.06124μg·g^(-1))、AsB(0.0031~0.0979μg·g^(-1))和DMA(0.0012~0.0070μg·g^(-1))为主,而这几种砷普遍被认为是低毒的。因此,正常膳食贝类引起的砷健康风险属于低关注级别。本研究有助于揭示人群日常摄食富砷贝类后砷在生物体内的累积分布及其代谢转化情况,同时也为人群正常摄食贝类的砷健康风险提供参考数据。The accumulation,metabolism,and transformation of arsenic in the organism were researched in a 14 d consecutive shellfish exposure experiment in mice.The results showed that after 14 d of dietary exposure,about 70%of the total arsenic ingested was finally excreted in feces.The accumulation of total arsenic in organs/tissues was as follows:stomach(0.26~0.54μg·g^(-1))>intestine(0.12~0.42μg·g^(-1))>spleen(0.031~0.162μg·g^(-1))>kidney(0.06~0.12μg·g^(-1))>liver(0.049~0.091μg·g^(-1)),with insignificant accumulation in the remaining organs/tissues(0.016~0.065μg·g^(-1)).About 46%of arsenobetaine(AsB)in food was excreted in feces,whereas about 576%of dimethylarsenic acid(DMA)was excreted.The content of DMA excreted was much higher than that ingested from the food,and thus arsenic methylation occurred in mice.Arsenate(AsⅤ)was only detected in the intestine,but the content(0.0054~0.0116μg·g^(-1))was very low.The accumulation of arsenic compounds in the remaining organs/tissues was mainly dominated by arseniccholine(AsC)(0.0011~0.06124μg·g^(-1)),AsB(0.0031~0.0979μg·g^(-1)),and DMA(0.0012~0.0070μg·g^(-1)),which were generally considered to be low-toxicity.Therefore,the health risk of arsenic caused through normal dietary shellfish is at a low level of concern.This study helps to reveal the cumulative distribution of arsenic and its metabolic transformation in organisms after ingestion of arsenic-rich shellfish,and also provides reference data on the health risk of arsenic in populations with normal consumption of shellfish.

关 键 词：砷形态 小鼠 代谢分布 摄食暴露 贝类 

分 类 号：X171.5[环境科学与工程—环境科学]