河北省典型蔬菜地土壤中抗生素抗性基因的污染特征及其健康风险评估  

Pollution Characteristics and Health Risk Assessment of Antibiotic R esistance Genes in Typical Vegetable Field Soil in Hebei Province

作　　者：李银辉 刘韵超 郑子英 龚玉敏 刘红[1] 闫艺漫 胡亚宁 刘瑾 王凤花 LI Yinhui;LIU Yunchao;ZHENG Ziying;GONG Yumin;LIU Hong;YAN Yiman;HU Yaning;LIU Jin;WANG Fenghua(School of Geographical Sciences,Hebei Normal University/Hebei Key Laboratory of Environmental Change and Ecological Construction/Hebei Technology Innovation Center for Remote Sensing Identification of Environmental Change/Hebei Experimental Teaching Demonstrating Center of Geographical Science,Shijiazhuang 050024,China)

机构地区：[1]河北师范大学地理科学学院/河北省环境演变与生态建设重点实验室/河北省环境变化遥感识别技术创新中心/河北省地理科学实验教学示范中心,石家庄050024

出　　处：《生态毒理学报》2025年第2期297-309,共13页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金面上基金项目(42077358);河北省自然科学基金项目(D2020205021);河北师范大学科学基金项目(L2022B20)。

摘　　要：抗生素抗性基因(antibiotics resistance genes,ARGs)作为一种新型环境污染物,在不同环境介质中广泛存在并传播。目前,畜禽粪肥的大量施用,已导致蔬菜地土壤成为ARGs的重要储存库。基于此,本研究利用实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reaction,qPCR)技术,解析了河北省典型蔬菜地土壤中ARGs的分布特征。结合土壤理化性质,通过冗余分析(redundancy analysis,RDA)等方法,探讨了影响ARGs分布特征的主控因子。此外,利用健康风险评价模型,对蔬菜地土壤中ARGs的潜在健康风险进行了评估。qPCR结果表明,蔬菜地土壤中ARGs丰度范围为3.30×10^(7)~4.64×10^(9)copies·g^(-1)(干土)。施加畜禽粪肥的土壤中ARGs丰度1.25×10^(9)copies·g^(-1)(干土)显著高于施加化肥的土壤中ARGs丰度2.48×10^(8)copies·g^(-1)(干土)。施加猪粪的土壤中ARGs丰度4.64×10^(9)copies·g^(-1)(干土)显著高于施加鸡粪的土壤1.26×10^(9)copies·g^(-1)(干土)、施加羊粪的土壤9.12×10^(8)copies·g^(-1)(干土)和施加蚯蚓粪的土壤中ARGs丰度4.59×10^(8)copies·g^(-1)(干土)。土壤中intI1基因丰度范围为8.17×10^(4)~3.21×10^(9)copies·g^(-1)(干土),且与tetC、tetG、sul1、sul2等呈显著正相关关系,说明intI1基因在ARGs的传播扩散中起着重要作用。此外,土壤中ARGs丰度与有机质、总氮和总磷等理化性质显著正相关,土壤理化性质和粪肥施加量可解释39.91%的ARGs丰度变化。健康风险评价结果表明,施加粪肥土壤ARGs的人体暴露剂量(儿童1.67×10^(4)copies·g^(-1)·d^(-1),成人2.34×10^(3)copies·g^(-1)·d^(-1))显著高于施加化肥土壤ARGs的人体暴露剂量(儿童2.99×10^(3)copies·g^(-1)·d^(-1),成人4.19×10^(2)copies·g^(-1)·d^(-1)),其中,经口摄入高于呼吸吸入和皮肤接触途径进入人体的ARGs暴露剂量,表明施加粪肥增加了ARGs对人体的潜在健康风险。本研究结果可为蔬菜地土壤ARGs污染现状的Antibiotic resistance genes(ARGs),as a novel environmental pollutant,are widely distributed and spread across various environmental media.At present,the extensive application of livestock and poultry manure has resulted in vegetable soil becoming a significant reservoir of ARGs.In this study,quantitative real-time polymerase chain reaction(qPCR)technology was employed to analyze the distribution characteristics of ARGs in typical vegetable soil in Hebei Province.Based on the physical and chemical properties of soil,the main controlling factors affecting the distribution characteristics of ARGs were explored through redundancy analysis(RDA)and other methods.Moreover,a health risk assessment model was utilized to evaluate the potential health risks a ssociated with ARGs in vegetable soil.The qPCR results revealed that the abundance of ARGs in vegetable field soil ranged from 3.30×10^(7)to 4.64×10^(9)copies·g^(-1)(dry soil).The abundance of ARGs in soil treated with livestock and poultry manure 1.25×10^(9)copies·g^(-1)(dry soil)was significantly higher than that in soil treated with chemical fertilizer 2.48×10^(8)copies·g^(-1)(dry soil).Additionally,the abundance of ARGs varied among soil with pig manure 4.64×10^(9)copies·g^(-1)(dry soil)was significantly higher than that in soil with chicken manure 1.26×10^(9)copies·g^(-1)(dry soil),sheep manure 9.12×10^(8)copies·g^(-1)(dry soil)and earthworm manure 4.59×10^(8)copies·g^(-1)(dry soil).The abundance of intI1 gene in soil ranged from 8.17×10^(4)to 3.21×10^(9)copies·g^(-1)(dry soil)and was significantly p ositively correlated with tetC,tetG,sul1,sul2,etc.,indicating its crucial role in facilitating the spread of ARGs.In addition,the abundance of ARGs in soil was significantly positively correlated with organic matter,total nitrogen,and total phosphorus,and the soil physicochemical properties and manure application rates accounted for 39.91%of the variation in ARG abundance.Furthermore,the health risk assessment results showed that the human e xposure

关 键 词：抗生素抗性基因 蔬菜地土壤 健康风险评估 粪肥 intI1基因 

分 类 号：X171.5[环境科学与工程—环境科学]